IVF Unit, Department of Obstetrics and Gynecology, Hillel-Yaffe Medical Center, Hadera 3820302, Israel.
A Ruth and Bruce Rappaport School of Medicine, Technion-Israel Institute of Technology, Haifa 3525433, Israel.
Cells. 2024 Nov 12;13(22):1872. doi: 10.3390/cells13221872.
This prospective, case-control study evaluated the impact of obesity on oocyte quality based on mtDNA expression in cumulus cells (CC), and on bone morphogenetic protein 15 (BMP-15) and heparan sulfate proteoglycan 2 (HSPG2) in follicular fluid (FF). It included women 18 to <40 years of age, divided according to BMI < 24.9 (Group 1, n = 28) and BMI > 25 (Group 2, n = 22). Demographics, treatment, and pregnancy outcomes were compared. The mtDNA in CC, BMP-15, HSPG2, the lipid profile, the hormonal profile, and C-reactive protein were evaluated in FF and in blood samples. The BMP-15 levels in FF and the mitochondrial DNA in CC were higher in Group 1 (38.8 ± 32.5 vs. 14.3 ± 10.8 ng/mL; = 0.001 and 1.10 ± 0.3 vs. 0.87 ± 0.18-fold change; = 0.016, respectively) than in Group 2. High-density lipoprotein levels in blood and FF were higher in Group 1 (62 ± 18 vs. 50 ± 12 mg/dL; = 0.015 and 34 ± 26 vs. 20.9 ± 7.2 mg/dL; = 0.05, respectively). Group 2 had higher blood C-reactive protein (7.1 ± 5.4 vs. 3.4 ± 4.3 mg/L; = 0.015), FF (5.2 ± 3.8 vs. 1.5 ± 1.6 mg/L; = 0.002) and low-density lipoprotein levels (91 ± 27 vs. 71 ± 22 mg/dL; = 0.008) vs. Group 1. Group 1 demonstrated a trend toward a better clinical pregnancy rate (47.8% vs. 28.6%: = 0.31) and frozen embryo transfer rate (69.2% vs. 53.8; = 0.69). Higher BMI resulted in lower BMP-15 levels and reduced mtDNA expression, which reflect decreased oocyte quality in overweight women.
本前瞻性病例对照研究评估了肥胖对卵母细胞质量的影响，其依据是卵丘细胞（CC）中的线粒体 DNA 表达，以及卵泡液（FF）中的骨形态发生蛋白 15（BMP-15）和硫酸乙酰肝素蛋白多糖 2（HSPG2）。研究纳入了 18 至<40 岁的女性，根据 BMI<24.9（第 1 组，n=28）和 BMI>25（第 2 组，n=22）进行分组。比较了两组的人口统计学、治疗和妊娠结局。评估了 CC 中的 mtDNA、BMP-15、HSPG2、血脂谱、激素谱和 C 反应蛋白在 FF 和血样中的水平。第 1 组 FF 中的 BMP-15 水平和 CC 中的线粒体 DNA 分别高于第 2 组（38.8±32.5 与 14.3±10.8ng/ml；=0.001 和 1.10±0.3 与 0.87±0.18 倍变化；=0.016）。第 1 组血和 FF 中的高密度脂蛋白水平较高（62±18 与 50±12mg/dL；=0.015 和 34±26 与 20.9±7.2mg/dL；=0.05）。第 2 组血 C 反应蛋白（7.1±5.4 与 3.4±4.3mg/L；=0.015）、FF（5.2±3.8 与 1.5±1.6mg/L；=0.002）和低密度脂蛋白水平（91±27 与 71±22mg/dL；=0.008）均高于第 1 组。第 1 组的临床妊娠率（47.8%与 28.6%：=0.31）和冷冻胚胎移植率（69.2%与 53.8%；=0.69）均呈上升趋势。较高的 BMI 导致 BMP-15 水平降低和 mtDNA 表达减少，这反映了超重女性卵母细胞质量下降。
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