Wu Yan-Ting, Tang Li, Cai Jie, Lu Xiu-E, Xu Jian, Zhu Xiao-Ming, Luo Qiong, Huang He-Feng
Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
Hum Reprod. 2007 Jun;22(6):1526-31. doi: 10.1093/humrep/dem029. Epub 2007 Mar 8.
Bone morphogenetic protein-15 (BMP-15) has been shown to influence oocyte maturation and quality. However, no relationship has been established between BMP-15 and oocyte quality/embryonic development in humans. The aim of this study is to investigate BMP-15 level in human follicular fluid (FF) and its possible role in determining oocyte quality and developmental potential.
A total of 79 oocytes and their corresponding FF from 79 women undergoing ICSI were examined. Individual oocytes were inseminated and subsequently assessed on the basis of their fertilization, cleavage and preimplantation development. BMP-15, FSH, estradiol (E(2)) and progesterone levels of FF were also analysed via the techniques of western blot or radioimmunoassay.
Higher FF BMP-15 levels were observed in the fertilized and cleaved groups versus the unfertilized and uncleaved groups, respectively (P < 0.05). The best (Grade I) embryo morphology was associated with higher FF BMP-15 levels than Grade II or III embryos (P < 0.01). A significant positive correlation was found between BMP-15 and E(2) levels in the same follicle.
The present study demonstrates that the BMP-15 level in FF appears to be a potential factor in predicting oocyte quality and subsequent embryo development, and is correlated with E(2) level, which may additionally be a valuable predictor of oocyte fertilization.
骨形态发生蛋白-15（BMP-15）已被证明会影响卵母细胞成熟和质量。然而，在人类中，BMP-15与卵母细胞质量/胚胎发育之间尚未建立联系。本研究的目的是调查人卵泡液（FF）中BMP-15水平及其在确定卵母细胞质量和发育潜力方面的可能作用。
对79例接受卵胞浆内单精子注射（ICSI）的女性的79个卵母细胞及其相应的卵泡液进行了检查。对单个卵母细胞进行授精，随后根据其受精、分裂和植入前发育情况进行评估。还通过蛋白质免疫印迹或放射免疫测定技术分析了卵泡液中BMP-15、促卵泡激素（FSH）、雌二醇（E₂）和孕酮水平。
受精组和分裂组的卵泡液BMP-15水平分别高于未受精组和未分裂组（P<0.05）。最佳（I级）胚胎形态与卵泡液中较高的BMP-15水平相关，高于II级或III级胚胎（P<0.01）。在同一卵泡中，BMP-15与E₂水平之间存在显著正相关。
本研究表明，卵泡液中BMP-15水平似乎是预测卵母细胞质量和后续胚胎发育的一个潜在因素，并且与E₂水平相关，E₂水平可能也是卵母细胞受精的一个有价值的预测指标。
