Kruse F E, Schmitz W, Jaeger W, Götz M L
Klin Monbl Augenheilkd. 1986 Jan;188(1):29-32. doi: 10.1055/s-2008-1050568.
Impression cytology specimens of the conjunctiva can be examined by scanning electron microscopy. If a cellulose-acetate filter is pressed onto the conjunctiva, the microvilli-bearing epithelium becomes so firmly attached to the fine meshwork of the filter that the most superficial conjunctival cell layers adhere to the filter. When the filter is removed intercellular connections are destroyed without any damage to the cell membranes being detectable in the SEM image. Since the surface of the corneal epithelium is spread out on the filter and is firmly attached to it the lateral bond between the cells is preserved. Shrinkage is minimal. The posterior surface of the epithelial cells, facing the observer, consists of smooth, porous cell membranes. The nuclei of many cells are clearly distinguishable. Since it can be directly compared with light-microscopic specimens, the SEM image can help provide answers to morphological and pathological questions, and may represent a useful diagnostic aid.
结膜印片细胞学标本可通过扫描电子显微镜进行检查。如果将醋酸纤维素滤膜压在结膜上，带有微绒毛的上皮会牢固地附着在滤膜的精细网眼上，以至于结膜最表层的细胞层会附着在滤膜上。当滤膜被取下时，细胞间连接被破坏，而在扫描电镜图像中未检测到细胞膜有任何损伤。由于角膜上皮表面铺展在滤膜上并与之牢固附着，细胞间的侧向连接得以保留。收缩极小。上皮细胞面向观察者的后表面由光滑、多孔的细胞膜组成。许多细胞的细胞核清晰可辨。由于扫描电镜图像可直接与光学显微镜标本进行比较，它有助于回答形态学和病理学问题，可能是一种有用的诊断辅助手段。
