Renard G, Pouliquen Y, Savoldelli M
J Fr Ophtalmol. 1980;3(10):571-8.
Transfixing keratoplasty was performed in a patient seven years after a lens extraction, following the appearance of corneal edema with hypertony and proliferation of a retrocorneal veil. Scanning electron microscopy examination of the posterior surface of the removed graft demonstrated the presence of a vast cellular veil formed of epithelial cells. The principal characteristics of these cells were their polygonal shape, slightly raised edges, and the presence of numerous surface microvilli. Because of the particular characteristics of this epithelium, scanning electron microscopy can be used to observe mitoses in the deep layer as well as for differentiation of the superficial layers. The epithelial cell appears to be identical, as far as its evolution is concerned, both in the aqueous humor and when in contact with the lacrymal film. When compared with other modern investigational techniques, the scanning electron microscope appears to be an effective method for studying epithelial invasion of the anterior chamber. In fact, optical microscopy is of little value in such cases, and transmitted light electron microscopy too heavy a technique for the results expected. Scanning electron microscopy enables precise definition of epithelial cells and can confirm their corneal or conjunctival origin.
在晶状体摘除术后七年,一名患者出现角膜水肿伴眼压升高及后弹力层增殖,遂行穿透性角膜移植术。对移除的移植片后表面进行扫描电子显微镜检查,发现存在由上皮细胞形成的巨大细胞层。这些细胞的主要特征是多边形形状、边缘略凸起以及存在大量表面微绒毛。由于这种上皮细胞的特殊特征,扫描电子显微镜可用于观察深层的有丝分裂以及表层的分化。就其演变而言,上皮细胞在房水中和与泪膜接触时似乎是相同的。与其他现代研究技术相比,扫描电子显微镜似乎是研究前房上皮细胞侵入的有效方法。事实上,光学显微镜在这种情况下价值不大,而透射光电子显微镜技术对于预期结果来说过于繁琐。扫描电子显微镜能够精确界定上皮细胞,并可确认其角膜或结膜来源。