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没食子酸通过P53/Bax信号通路诱导HeLa细胞系凋亡。

Gallic Acid Induces HeLa Cell Lines Apoptosis via the P53/Bax Signaling Pathway.

作者信息

Sarı Umut, Zaman Fuat, Özdemir İlhan, Öztürk Şamil, Tuncer Mehmet Cudi

机构信息

Department of Gynecology and Obstetrics, Umut Sarı Clinic, 34371 Istanbul, Turkey.

Department of Obstetrics and Gynecology, Diyarlife Hospital, 21100 Diyarbakır, Turkey.

出版信息

Biomedicines. 2024 Nov 18;12(11):2632. doi: 10.3390/biomedicines12112632.

DOI:10.3390/biomedicines12112632
PMID:39595196
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11591794/
Abstract

BACKGROUND

Cervical cancer is a type of cancer that originates from the endometrium and is more common in developed countries and its incidence is increasing day by day in developing countries. The most commonly prescribed chemotherapeutic drugs limit their use due to serious side effects and the development of drug resistance. For this reason, interest in new active ingredients obtained from natural products is increasing. This study aimed to reveal the apoptotic and antiproliferative effects of gallic acid and doxorubicin combination therapy against the HeLa cell line.

METHODS

We investigated the anti-cancer effects of doxorubicin and gallic acid in the human HeLa cervical cell line by using the MTT test, Nucblue staining for the identification of apoptotic cells due to nuclear condensation using fluorescent substance, and apoptotic markers P53 and Bax for the RT-PCR test.

RESULTS

The highest cytotoxic effect obtained in the study, the highest increase in apoptotic induction, and a significant difference in P53/Bax levels were seen in the gallic acid/doxorubicin combination. Additionally, it was determined that gallic acid exhibited an effective cytotoxic effect on HeLa and HaCat cells within 48 and 72 h of application.

CONCLUSIONS

The obtained findings show that the gallic acid/doxorubicin combination applied to HeLa cells may be an alternative treatment against both the cytotoxic effect size and the side effects of the chemotherapy agent.

摘要

背景

宫颈癌是一种起源于子宫内膜的癌症，在发达国家更为常见，且在发展中国家其发病率日益上升。最常用的化疗药物由于严重的副作用和耐药性的产生而限制了其使用。因此，对从天然产物中获得的新活性成分的兴趣与日俱增。本研究旨在揭示没食子酸与阿霉素联合治疗对人宫颈癌细胞系HeLa的凋亡和抗增殖作用。

方法

我们通过MTT试验、使用荧光物质进行核浓缩鉴定凋亡细胞的Nucblue染色以及用于RT-PCR试验的凋亡标志物P53和Bax，研究了阿霉素和没食子酸对人HeLa宫颈癌细胞系的抗癌作用。

结果

在没食子酸/阿霉素联合用药组中观察到了研究中获得的最高细胞毒性作用、凋亡诱导的最高增加以及P53/Bax水平的显著差异。此外，还确定没食子酸在应用48小时和72小时内对HeLa和HaCat细胞表现出有效的细胞毒性作用。

结论

所得结果表明，应用于HeLa细胞的没食子酸/阿霉素联合用药可能是一种针对化疗药物的细胞毒性作用大小和副作用的替代治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/2d206371e1d6/biomedicines-12-02632-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/d54a62a74e9d/biomedicines-12-02632-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/5f151edb5b09/biomedicines-12-02632-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/8a10c8090322/biomedicines-12-02632-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/5e3aebec221c/biomedicines-12-02632-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/6bda3f6c868d/biomedicines-12-02632-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/7a0a3c081255/biomedicines-12-02632-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/f3904114b78d/biomedicines-12-02632-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/2d206371e1d6/biomedicines-12-02632-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/d54a62a74e9d/biomedicines-12-02632-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/5f151edb5b09/biomedicines-12-02632-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/8a10c8090322/biomedicines-12-02632-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/5e3aebec221c/biomedicines-12-02632-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/6bda3f6c868d/biomedicines-12-02632-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/7a0a3c081255/biomedicines-12-02632-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/f3904114b78d/biomedicines-12-02632-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/918c/11591794/2d206371e1d6/biomedicines-12-02632-g008.jpg

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