Rodrigues-Jesus Joana, Canadas-Sousa Ana, Santos Marta, Oliveira Pedro, Figueira Ana Catarina, Marrinhas Carla, Petrucci Gonçalo N, Gregório Hugo, Tinoco Flora, Goulart Andrea, Felga Helena, Vilhena Hugo, Dias-Pereira Patrícia
Department of Pathology and Molecular Immunology, School of Medicine and Biomedical Sciences, ICBAS-UP, University of Porto, 4050-313 Porto, Portugal.
Centre for Investigation Vasco da Gama (CIVG), Department of Veterinary Sciences, Vasco da Gama University School, 3020-210 Coimbra, Portugal.
Animals (Basel). 2024 Nov 14;14(22):3280. doi: 10.3390/ani14223280.
Necrosis is a common finding in human and animal neoplasms. The percentage of tumor necrosis is included in tumor grading schemes in veterinary oncology; however, evaluation methods are often overlooked. Different studies have assessed the prognostic value of tumor necrosis in feline mammary tumors with contradictory results, which could be related to methodologic variability. In this study, a comprehensive evaluation of tumor necrosis in feline mammary tumors (FMTs) was conducted, by applying a semi-quantitative and a quantitative methodology for assessing necrosis. The interobserver agreement, the relationship with clinicopathological characteristics and the prognostic value of necrosis were analyzed in 154 FMT cases. Although subjectivity in the assessment of necrosis existed, an almost perfect agreement (weighted quadratic k = 0.851) between two observers was obtained. Furthermore, there was a significant positive correlation between the semi-quantitative and quantitative methods. Necrosis was more common and more extensive in malignant tumors than in their benign counterparts. Despite the non-significant results in the survival analysis, extensive necrosis was significantly associated with aggressive clinicopathological features, such as higher histological grade, high mitotic count and lymphovascular invasion. Our results support the potential relevance of necrosis in FMT.
坏死是人和动物肿瘤中的常见表现。肿瘤坏死的百分比被纳入兽医肿瘤学的肿瘤分级方案中；然而，评估方法常常被忽视。不同的研究评估了猫乳腺肿瘤中肿瘤坏死的预后价值，但结果相互矛盾，这可能与方法的变异性有关。在本研究中，通过应用半定量和定量方法评估坏死，对猫乳腺肿瘤（FMT）中的肿瘤坏死进行了全面评估。在154例FMT病例中分析了观察者间的一致性、坏死与临床病理特征的关系以及坏死的预后价值。虽然在坏死评估中存在主观性，但两名观察者之间获得了几乎完美的一致性（加权二次kappa = 0.851）。此外，半定量和定量方法之间存在显著的正相关。坏死在恶性肿瘤中比在良性肿瘤中更常见且更广泛。尽管生存分析结果不显著，但广泛坏死与侵袭性临床病理特征显著相关，如更高的组织学分级、高有丝分裂计数和淋巴管浸润。我们的结果支持坏死在FMT中的潜在相关性。
