Venkatraman Anumitha, Bretl Michelle, Kim Se-In, Christensen Leslie, Kelm-Nelson Cynthia A, Ciucci Michelle R, Thibeault Susan L
Division of Otolaryngology-Head & Neck Surgery, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA.
Ebling Library for the Health Sciences, University of Wisconsin-Madison, Madison, WI 53705, USA.
Brain Sci. 2024 Oct 31;14(11):1109. doi: 10.3390/brainsci14111109.
: Ultrasonic vocalization (USV) can indicate affective states-including psychosocial stress-in mice and rats. However, stress-induced USV changes could be confounded by laboratory experimental variables such as the type of behavioral stress paradigm, the elicitation method, rodent strain, etc. We sought to provide a review of the current literature to delineate how psychosocial stress-altered rodent USVs may be affected by factors of age, sex, strain, species, elicitation paradigm, and stressor. : We used PubMed, Scopus (Elsevier), PsycINFO (EBSCO), and the following Web of Science (Clarivate) databases: Biological Abstracts, CAB Abstracts, Science Citation Index-Expanded, and Emerging Sources Citation Index. The studies identified by our search strategy were independently screened by two authors with the following inclusion criteria: peer-reviewed, in English, reported original data, and described USV in response to stress in rats or mice. The data extracted included USV acoustic parameters (mean peak frequency and mean amplitude (loudness)), details of the stress and USV elicitation paradigms, rodent species, age, and sex variables. : The following screening of 5309 titles/abstracts and 687 full-text articles revealed 148 articles. Footshock (20%), cold exposure (14%), and maternal separation (23.5%) were the most commonly used stress paradigms (duration and type of stressor varied across studies), with the total number of USV calls being the most commonly reported acoustic outcome. In rats, 121 articles described stress-altered USVs, while 25 studies reported the same in mice, and two reported multiple rodent species (rats and mice, alongside other rodent species such as gerbils). With respect to stress-altered USV changes with age, mice and rats increase USV rates after birth, with a peak around 6 to 10 days, and decrease USVs until weanling age. Of the five studies that reported sex-related differences in stress-induced USVs, females had an increased number of calls and lower average peak frequency in response to stress when compared to males. Only two to four studies reported strain-related differences in stress-induced vocalizations in rats and mice, respectively. : The data from this review lay the groundwork for better understanding rodent USVs in response to psychosocial stress with effects of elicitation paradigm, stressor, age, and sex.
超声波发声（USV）可表明小鼠和大鼠的情绪状态，包括心理社会压力。然而，压力诱导的USV变化可能会受到实验室实验变量的混淆，如行为应激范式的类型、诱发方法、啮齿动物品系等。我们试图对当前文献进行综述，以阐明心理社会压力改变的啮齿动物USV如何受到年龄、性别、品系、物种、诱发范式和应激源等因素的影响。
我们使用了PubMed、Scopus（爱思唯尔）、PsycINFO（EBSCO）以及以下Web of Science（科睿唯安）数据库：生物学文摘、CAB文摘、科学引文索引扩展版和新兴来源引文索引。通过我们的搜索策略确定的研究由两位作者根据以下纳入标准独立筛选：同行评审、英文、报告原始数据，并描述大鼠或小鼠对应激的USV。提取的数据包括USV声学参数（平均峰值频率和平均振幅（响度））、应激和USV诱发范式的细节、啮齿动物物种、年龄和性别变量。
对5309篇标题/摘要和687篇全文文章的以下筛选共得到148篇文章。足部电击（20%）、冷暴露（14%）和母婴分离（23.5%）是最常用的应激范式（应激源的持续时间和类型因研究而异），USV叫声总数是最常报告的声学结果。在大鼠中，121篇文章描述了应激改变的USV，而25项研究报告了小鼠的相同情况，两项研究报告了多种啮齿动物物种（大鼠和小鼠，以及沙鼠等其他啮齿动物物种）。关于应激改变的USV随年龄的变化，小鼠和大鼠出生后USV率增加，在6至10天左右达到峰值，断奶前USV率下降。在五项报告应激诱导的USV存在性别差异的研究中，与雄性相比，雌性对应激的叫声数量增加且平均峰值频率较低。分别只有两到四项研究报告了大鼠和小鼠应激诱导发声的品系差异。
本综述的数据为更好地理解啮齿动物在心理社会压力下的USV奠定了基础，这些压力受到诱发范式、应激源、年龄和性别的影响。
