Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China, Guizhou Academy of Forestry, Guiyang 550005, China.
Guizhou Liping Rocky Desertification Ecosystem Observation and Research Station, Liping 556200, China.
Int J Mol Sci. 2024 Nov 7;25(22):11973. doi: 10.3390/ijms252211973.
, a widely cultivated woody oil crop, holds economic significance because of its ability to grow without encroaching on cultivated land. The pericarp of is abundant in flavonoids and phenolic acids, which offer significant nutritional benefits. This study used metabolomic technology (UPLC-ESI-MS/MS) to discern metabolite variances in the pericarp of three types (COT, BFOT, and SFOT) during the maturity stage and subsequently analyzed and compared them. A total of 1117 metabolites were detected in the study, including 277 flavonoids, 221 phenolic acids, 108 lipids, 93 amino acids and their derivatives, 83 organic acids, 59 nucleotides and their derivatives, 57 alkaloids, 52 lignans, 44 tannins, 23 terpenoids, and 100 miscellaneous metabolites (such as sugars, alcohols, vitamins, and other unclassified substances). Clustering and PCA analyses revealed distinct separation of COT, BFOT, and SFOT, indicating variances in metabolites within the pericarp peels of these three types. KEGG enrichment analysis demonstrated that 143 shared differential metabolites were primarily associated with amino acid biosynthesis. These findings are expected to significantly enhance the current knowledge of the pericarp and pave the way for future development and use efforts.
油桐，一种广泛种植的木本油料作物，因其能够在不侵占耕地的情况下生长而具有经济意义。油桐果皮富含类黄酮和酚酸，具有显著的营养价值。本研究采用代谢组学技术（UPLC-ESI-MS/MS）在成熟阶段辨别三种油桐（COT、BFOT 和 SFOT）果皮中的代谢物差异，并对其进行分析和比较。在研究中检测到 1117 种代谢物，包括 277 种类黄酮、221 种酚酸、108 种脂质、93 种氨基酸及其衍生物、83 种有机酸、59 种核苷酸及其衍生物、57 种生物碱、52 种木脂素、44 种单宁、23 种萜类化合物和 100 种其他代谢物（如糖、醇、维生素和其他未分类物质）。聚类和 PCA 分析表明，COT、BFOT 和 SFOT 之间存在明显的分离，表明这三种油桐果皮内的代谢物存在差异。KEGG 富集分析表明，143 个共有差异代谢物主要与氨基酸生物合成有关。这些发现有望极大地提高对油桐果皮的现有认识，并为未来的开发和利用工作铺平道路。
