Jiangsu Academy of Forestry, 109 Danyang Road, Dongshanqiao, Nanjing 211153, China.
Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of State Forestry and Grassland Administration on Subtropical Forest Biodiversity Conservation, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2024 Nov 8;25(22):12027. doi: 10.3390/ijms252212027.
is a valuable plant used both as food and medicine in China, but low production limits the development of its industry. As such, it is important to develop genetic resources for the high-value species for preservation of wild populations and utilization. The complete chloroplast and nuclear genomes have already been available; however, its mitogenome has not yet been characterized. Here, the mitogenome was assembled using HiFi reads, and a comparative analysis was conducted. The mitogenome was a circular sequence of 708,384 bp with a GC content of 45.28%, in which 67 genes were annotated, including 36 protein-coding genes, 26 tRNA genes, 3 rRNA genes, and 2 pseudogenes. Overall, 370 dispersed repeats, 161 simple repeats, and 42 tandem repeats were identified, and 360 RNA editing sites were predicted. There was extensive DNA migration among the three genomes. In addition, most of the protein-coding genes underwent purifying selection throughout evolution, and the nucleotide diversity was highly variable. In addition, comparative analysis indicated that the sizes, structures, and gene contents of the mitogenomes differed significantly, but the GC contents and functional genes were relatively conserved among the Ericales species. Mitogenome-based phylogenetic analysis indicated the precise. evolutionary and taxonomic status of . The complete mitogenome represents the last link of the reference genome of and lays the foundation for effective utilization and molecular breeding of this plant.
是中国一种有价值的植物，既可用作食物，也可用作药物，但由于产量低，限制了其产业的发展。因此，开发高价值物种的遗传资源对于保护野生种群和利用具有重要意义。该物种的完整叶绿体和核基因组已经可用，但线粒体基因组尚未得到描述。在这里，使用 HiFi 读取组装了该物种的线粒体基因组，并进行了比较分析。线粒体基因组是一个圆形序列，大小为 708,384 bp，GC 含量为 45.28%，其中注释了 67 个基因，包括 36 个蛋白质编码基因、26 个 tRNA 基因、3 个 rRNA 基因和 2 个假基因。总的来说，鉴定出了 370 个分散重复序列、161 个简单重复序列和 42 个串联重复序列，并预测了 360 个 RNA 编辑位点。三个基因组之间存在广泛的 DNA 迁移。此外，大多数蛋白质编码基因在进化过程中经历了纯化选择，核苷酸多样性高度可变。此外，比较分析表明，线粒体基因组的大小、结构和基因含量差异显著，但在桃金娘目物种中，GC 含量和功能基因相对保守。基于线粒体基因组的系统发育分析表明，该物种的进化和分类地位准确。完整的线粒体基因组代表了该植物参考基因组的最后一环，为该植物的有效利用和分子育种奠定了基础。
