Faculty of Veterinary Medicine, Department of Pathology, Division of Microbiology, Wrocław University of Environmental and Life Sciences, 50-375 Wrocław, Poland.
Department of Physiology in Zabrze, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland.
Int J Mol Sci. 2024 Nov 11;25(22):12088. doi: 10.3390/ijms252212088.
Respiratory viruses are among the most common causes of human infections. Examining pathological processes linked to respiratory viral infections is essential for diagnosis, treatment strategies, and developing novel therapeutics. Alterations in oxidative stress levels and homeostasis are significant processes associated with respiratory viral infections. The study aimed to compare selected oxidative stress markers: total oxidative status (TOS), total antioxidant capacity (TAC), and the oxidative stress index (OSI) levels and glutathione peroxidase (GPx) and glutathione reductase (GR) activities in normal (MRC5 cell line) and tumor (A549 cell line) lung cells infected with human coronaviruses (HCoV) OC43 and 229E, human adenovirus type 5 (HAdV5), or human rhinovirus A (HRV A). We observed that a respiratory viral infection more significantly affected non-enzymatic oxidative stress markers in a lung adenocarcinoma model (A549 cells), while human lung fibroblasts (MRC-5 cell line) presented changes in enzymatic and non-enzymatic oxidative stress markers. We suggest that further detailed research is required to analyze this phenomenon.
呼吸道病毒是人类感染最常见的原因之一。研究与呼吸道病毒感染相关的病理过程对于诊断、治疗策略和开发新的治疗方法至关重要。氧化应激水平和内稳态的改变是与呼吸道病毒感染相关的重要过程。本研究旨在比较正常（MRC5 细胞系）和肿瘤（A549 细胞系）肺细胞在感染人冠状病毒（HCoV）OC43 和 229E、人腺病毒 5 型（HAdV5）或人鼻病毒 A（HRV A）后，选择的氧化应激标志物：总氧化状态（TOS）、总抗氧化能力（TAC）和氧化应激指数（OSI）水平以及谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）活性的变化。我们观察到，呼吸道病毒感染更显著地影响了肺腺癌细胞模型（A549 细胞）中非酶促氧化应激标志物，而人肺成纤维细胞（MRC-5 细胞系）则表现出酶促和非酶促氧化应激标志物的变化。我们建议需要进一步进行详细的研究来分析这种现象。
