School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510641, China.
National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food, Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China.
Int J Mol Sci. 2024 Nov 12;25(22):12116. doi: 10.3390/ijms252212116.
() is a zoonotic foodborne pathogen that is widely distributed worldwide. Its optimal growth environment is microaerophilic conditions (5% O, 10% CO), but it can spread widely in the atmospheric environment. Biofilms are thought to play an important role in this process. However, there are currently relatively few research works on the regulatory mechanisms of biofilm formation. In this study, a pan-genome analysis, combined with the analysis of biofilm phenotypic information, revealed that the gene cluster - is associated with the negative regulation of biofilm formation in Through gene knockout experiments, it was observed that the - mutant strain significantly increased biofilm formation and enhanced flagella formation. Furthermore, pull-down assay revealed that Cj0424 interacts with 93 proteins involved in pathways such as fatty acid synthesis and amino acid metabolism, and it also contains the quorum sensing-related gene This suggests that - affects fatty acid synthesis and amino acid metabolism, influencing quorum sensing and strain motility, ultimately inhibiting biofilm formation.
（）是一种人畜共患的食源性致病菌，广泛分布于世界各地。其最佳生长环境为微需氧条件（5%O、10%CO），但它可以在大气环境中广泛传播。生物膜被认为在此过程中发挥着重要作用。然而，目前关于生物膜形成的调控机制的研究工作相对较少。在这项研究中，通过全基因组分析，结合生物膜表型信息分析，揭示了基因簇 - 与 生物膜形成的负调控有关。通过基因敲除实验，观察到 - 突变株的生物膜形成显著增加，并增强了鞭毛形成。此外，下拉实验表明 Cj0424 与参与脂肪酸合成和氨基酸代谢等途径的 93 种蛋白相互作用，它还包含群体感应相关基因 这表明 - 影响脂肪酸合成和氨基酸代谢，影响群体感应和菌株运动性，最终抑制生物膜形成。
