State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China.
Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
Int J Mol Sci. 2024 Nov 13;25(22):12172. doi: 10.3390/ijms252212172.
GET3 is an ATPase protein that plays a pivotal role in the guided entry of the tail-anchored (GET) pathway. The protein facilitates the targeting and inserting of tail-anchored (TA) proteins into the endoplasmic reticulum (ER) by interacting with a receptor protein complex on the ER. The role of GET3 in various biological processes has been established in yeast, plants, and mammals but not in filamentous fungi. is the major causal agent of Fusarium head blight (FHB), posing a threat to the yield and quality of wheat. In this study, we found that FgGET3 exhibits a high degree of sequence and structural conservation with its homologs across a wide range of organisms. Ectopic expression of in yeast restored the growth defects of the knock-out mutant. Furthermore, FgGET3 was found to dimerize and localize to the cytoplasm, similar to its homologs in other species. Deletion of in results in decreased fungal growth, fragmented vacuoles, altered abiotic stress responses, reduced conidia production, delayed conidial germination, weakened virulence on wheat spikes and reduced DON production. Collectively, these findings underscore the critical role of in regulating diverse cellular and biological functions essential for the growth and virulence of .
GET3 是一种 ATP 酶蛋白，在尾部锚定 (GET) 途径的靶向进入中发挥关键作用。该蛋白通过与内质网 (ER) 上的受体蛋白复合物相互作用，促进尾部锚定 (TA) 蛋白靶向和插入内质网。GET3 在酵母、植物和哺乳动物中的各种生物学过程中的作用已得到证实，但在丝状真菌中尚未得到证实。 是赤霉病（FHB）的主要致病因子，对小麦的产量和质量构成威胁。在本研究中，我们发现 FgGET3 与广泛的生物体中的同源物具有高度的序列和结构保守性。在酵母中异位表达 可恢复 敲除突变体的生长缺陷。此外，发现 FgGET3 二聚化并定位于细胞质中，类似于其他物种中的同源物。在 中缺失 会导致真菌生长减少、液泡碎片化、非生物胁迫反应改变、分生孢子产生减少、分生孢子萌发延迟、对小麦穗的毒力减弱以及 DON 产量降低。总之，这些发现强调了 对于调节各种细胞和生物学功能的关键作用，这些功能对于 的生长和毒力至关重要。
