Key Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin 150040, China.
College of Life Science, Northeast Forestry University, Harbin 150040, China.
Int J Mol Sci. 2024 Nov 17;25(22):12349. doi: 10.3390/ijms252212349.
Soil salinization is a significant environmental stress factor, threatening global agricultural yield and ecological security. Plants must effectively cope with the adverse effects of salt stress on survival and successful reproduction. Lateral Organ Boundaries (LOB) Domain (LBD) genes, a gene family encoding plant-specific transcription factors (TFs), play important roles in plant growth and development. Here, we identified and functionally characterized the LBD family TF PtrLBD41 from , which can be induced by various abiotic stresses, including salt, dehydration, low temperature, and Abscisic Acid (ABA). Meanwhile, transgenic plants overexpressing showed a better phenotype and higher tolerance than the wild-type (WT) plants under salt stress treatment. Transcriptome analysis found that the differentially expressed genes (DEGs) between the WT and overexpression (OE) line were enriched in the flavonoid biosynthetic process, in which chalcone synthases (CHS), naringenin 3-dioxygenase (F3H), and chalcone isomerase (CHI) were significantly up-regulated under salt stress conditions through qRT-PCR analysis. Therefore, we demonstrate that PtrLBD41 plays an important role in the tolerance to salt stress in .
土壤盐渍化是一个重要的环境胁迫因素，威胁着全球农业产量和生态安全。植物必须有效地应对盐胁迫对生存和成功繁殖的不利影响。侧生器官边界（LOB）结构域（LBD）基因是一类编码植物特异性转录因子（TFs）的基因家族，在植物生长和发育中发挥着重要作用。在这里，我们从 中鉴定并功能表征了 LBD 家族 TF PtrLBD41，它可以被各种非生物胁迫诱导，包括盐、脱水、低温和脱落酸（ABA）。同时，过表达 的转基因植物在盐胁迫处理下表现出比野生型（WT）植物更好的表型和更高的耐受性。转录组分析发现，WT 和过表达（OE）系之间的差异表达基因（DEGs）在类黄酮生物合成过程中富集，其中在盐胁迫条件下通过 qRT-PCR 分析发现查尔酮合酶（CHS）、柚皮素 3-双加氧酶（F3H）和查尔酮异构酶（CHI）显著上调。因此，我们证明 PtrLBD41 在 对盐胁迫的耐受性中发挥重要作用。
