State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin 150040, China; Bamboo Research Institute, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China; Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 51 Hexing Road, Harbin 150040, China.
Gene. 2019 Jul 30;707:189-197. doi: 10.1016/j.gene.2019.04.071. Epub 2019 Apr 25.
Transcription factor (TF) genes play essential roles in abiotic stress responses as master switches in complex regulatory networks. In the present study, the transcript abundance of 4287 TF genes in Populus simonii × P.nigra were profiled under NaCl, KCl, CdCl and PEG stresses, respectively. A total of 118 up-regulated and 226 down-regulated TFs were identified to be shared in the four stress conditions. Among the top seven TF families (ERF, NAC, WRKY, MYB, bHLH, C2H2, bZIP), there were 76 up-regulated TFs found common in the four stresses, and 67% of them were likely to be involved in stress responses. We identified three TFs, which can enhance stress tolerance of transgenic plants, were members of the most significantly up-regulated genes in the respective TF family. Among them, a highly salt-inducible ERF gene, ERF76, was proved to activate the expression of other TFs in the transgenic poplar lines overexpressing ERF76. Transcriptome analysis indicated there was a synergistic effect of TFs on improving salinity tolerance of the transgenic plants. Of significant interest in the study is the discovery of the role and interactions of various TF genes under multiple stress conditions.
转录因子（TF）基因作为复杂调控网络中的主开关，在非生物胁迫响应中发挥着重要作用。本研究分别在 NaCl、KCl、CdCl 和 PEG 胁迫下，对胡杨杂种（Populus simonii × P.nigra）中的 4287 个 TF 基因的转录丰度进行了分析。在这四种胁迫条件下，共鉴定到 118 个上调和 226 个下调的 TF。在排名前七的 TF 家族（ERF、NAC、WRKY、MYB、bHLH、C2H2、bZIP）中，有 76 个上调的 TF 在四种胁迫下共同存在，其中 67%可能参与了胁迫反应。我们鉴定出三个能够增强转基因植物耐胁迫能力的 TF，它们是各自 TF 家族中表达水平上调最显著的基因成员之一。其中，一个高度盐诱导的 ERF 基因 ERF76，被证明可以激活过表达 ERF76 的转基因杨树系中其他 TF 的表达。转录组分析表明，TF 对提高转基因植物的耐盐性有协同作用。本研究的一个重要发现是，在多种胁迫条件下，各种 TF 基因的作用和相互作用。
