College of Agronomy and Biotechnology, Southwest University, No. 2 Tiansheng Road, Beibei, Chongqing 400716, China.
Chongqing Academy of Agricultural Sciences, Nongke Road, Jiulongpo, Chongqing 401329, China.
Genes (Basel). 2024 Oct 24;15(11):1371. doi: 10.3390/genes15111371.
Rapeseed is an important commercial crop globally, used for both animal fodder and human consumption. Varied insolation duration and intensity are among the main factors affecting the seed yield and quality of () worldwide. In this study, the high-oil-content rapeseed cultivar "Qingyou 3" was subjected to a light supplementation trial during both the vegetative growth period and the seed productive stage. Different light intensity conditions were stimulated using light-emitting diodes (LEDs). The main plot factor was land condition, with LED treatment (Treatment) and without LED treatment (Control) under natural conditions. The results showed that the leaf size and thickness, photosynthesis efficiency, and seed oil content of increased significantly after light supplementation. Then, 18 cDNA libraries were constructed from leaf segments (30 days after transplanting-DAT) and seeds 30 and 40 days after pollination (DPA) for RNA transcriptome sequencing. It was found that genes encoding lipid transfer protein, phenylpropanoid biosynthesis, photosynthesis, and plant hormone signal transduction were enriched in differentially expressed genes (DEGs). The qRT-PCR analysis showed that eight key genes had significant variations, a finding also consistent with the RNA-seq results. The aim of this study was to identify the DEGs and signaling pathways in the leaves and seeds of during the vegetative and seed productive stages under different light intensities. The results provide insight into how sufficient light plays a critical role in promoting photosynthesis and serves as the foundation for material accumulation and yield formation.
油菜是全球重要的商业作物，既可用作动物饲料，也可食用。光照持续时间和强度的变化是影响全球油菜籽产量和品质的主要因素之一。本研究对高油油菜品种“青油 3 号”进行了光补充试验，分别在营养生长阶段和种子生产阶段进行。采用发光二极管（LED）刺激不同的光照强度条件。主区因素是土地条件，自然条件下有 LED 处理（处理）和无 LED 处理（对照）。结果表明，光照补充后，油菜叶片大小和厚度、光合作用效率和种子含油量均显著增加。然后，从叶片（移栽后 30 天-DAT）和授粉后 30 和 40 天（DPA）的种子构建了 18 个 cDNA 文库，进行 RNA 转录组测序。结果发现，编码脂质转移蛋白、苯丙烷生物合成、光合作用和植物激素信号转导的基因在差异表达基因（DEGs）中富集。qRT-PCR 分析显示，8 个关键基因有显著变化，与 RNA-seq 结果一致。本研究旨在鉴定不同光照强度下油菜营养生长和种子生产阶段叶片和种子中的 DEGs 和信号通路。研究结果表明，充足的光照在促进光合作用中起着至关重要的作用，为物质积累和产量形成提供了基础。
