Guangxi Marine Microbial Resources Industrialization Engineering Technology Research Center, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, 158 University Road, Nanning 530008, China.
Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
Genes (Basel). 2024 Nov 17;15(11):1479. doi: 10.3390/genes15111479.
Maintaining an optimum transport density is essential for protecting water quality, lowering stress levels, and increasing fish survival rates. Transporting marine fish fry involves major dangers. The purpose of this study was to evaluate the impact of transport stress at varying densities on the immune-related gene expression, antioxidant capacity, and survival rate of yellowfin seabream () fry. A 12 h simulated transport experiment was conducted with fry divided into six density groups. For 1-2 cm fry, densities of 900, 1200, and 1500 fry per pouch were used to assess antioxidant enzyme activity; and for 4-5 cm fry, densities of 100, 125, and 150 fry per pouch were used for gene expression analysis. The key parameters measured included survival rates, antioxidant enzyme activities in liver and intestinal tissues, and expression levels of and genes. The findings showed that recovery time and density both affected the observed responses and that transport density had a substantial effect on antioxidant enzyme activity in all tissues. The intestinal and liver tissues showed a considerable decrease in antioxidant enzyme activity, suggesting that these tissues may be able to respond to oxidative stress. Moreover, under high-density transport conditions, there were notable increases in the expression of and , suggesting the activation of immune response systems. This research offers valuable new understandings into the relationship between transport density and immunological and antioxidant modulation in fry. The results provide a scientific foundation for enhancing aquaculture transport conditions, which will ultimately lead to decreased fish mortality and improved general health during transit, resulting in more sustainable and effective aquaculture methods.
维持最佳运输密度对于保护水质、降低应激水平和提高鱼类存活率至关重要。运输海水鱼苗涉及重大危险。本研究旨在评估不同密度下的运输应激对黄鳍鲷鱼苗免疫相关基因表达、抗氧化能力和存活率的影响。对 1-2 厘米的鱼苗进行了 12 小时的模拟运输实验，将鱼苗分为六个密度组。对于 4-5 厘米的鱼苗，使用每袋 100、125 和 150 尾的密度进行基因表达分析。测量的关键参数包括存活率、肝脏和肠道组织中的抗氧化酶活性以及 和 基因的表达水平。结果表明，恢复时间和密度都会影响观察到的反应，并且运输密度对所有组织的抗氧化酶活性都有显著影响。肠道和肝脏组织中的抗氧化酶活性明显下降，表明这些组织可能能够应对氧化应激。此外，在高密度运输条件下， 和 的表达显著增加，表明免疫反应系统被激活。这项研究为了解运输密度与黄鳍鲷鱼苗免疫和抗氧化调节之间的关系提供了新的认识。研究结果为改善水产养殖运输条件提供了科学依据，最终将降低鱼类在运输过程中的死亡率，提高整体健康水平，实现更可持续和有效的水产养殖方法。
