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Evaluation of the NG-Test Carba 5 for the clinical detection of carbapenemase-producing gram-negative bacteria.评估NG-Test Carba 5用于临床检测产碳青霉烯酶革兰氏阴性菌的效果。
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An Evaluation of the ASTar Automated Antimicrobial Testing System for Gram-Negative Bacteria in Positive Blood Cultures.AStar自动抗菌检测系统对阳性血培养物中革兰氏阴性菌的评估
Antibiotics (Basel). 2025 Mar 1;14(3):249. doi: 10.3390/antibiotics14030249.

在阳性血培养物中使用NG-Test CARBA 5快速检测碳青霉烯酶：一项诊断试验研究

Rapid Detection of Carbapenemases Using NG-Test CARBA 5 in Positive Blood Cultures: A Diagnostic Test Study.

作者信息

Munguia-Ramos Diana, Xancal-Salvador Luis Fernando, Esteban-Kenel Verónica, Ortiz-Conchi Narciso, Jaimes-Aquino Ricardo Antonio, Mendoza-Rojas Miguel, Cervantes-Sánchez Axel, Méndez-Ramos Steven, Rivera-Villegas Hector Orlando, Rajme-Lopez Sandra, Tamez-Torres Karla Maria, Roman-Montes Carla Marina, Martínez-Gamboa Areli, Bobadilla Del-Valle Miriam, Sifuentes-Osornio Jose, Ponce-de-Leon Alfredo, Gonzalez-Lara Maria Fernanda, Martinez-Guerra Bernardo Alfonso

机构信息

Department of Infectious Diseases, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 15 Vasco de Quiroga, Belisario Domínguez Secc 16, Tlalpan, Mexico City 14080, Mexico.

Clinical Microbiology Laboratory, Department of Infectious Diseases, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, 15 Vasco de Quiroga, Belisario Domínguez Secc 16, Tlalpan, Mexico City 14080, Mexico.

出版信息

Antibiotics (Basel). 2024 Nov 20;13(11):1105. doi: 10.3390/antibiotics13111105.

DOI:10.3390/antibiotics13111105
PMID:39596798
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11591157/
Abstract

BACKGROUND

Infections due to carbapenem-resistant Gram-negative bacteria are emerging as an important challenge in health-care settings and a growing concern worldwide. Lateral flow immunoassay NG-Test CARBA 5 can detect the five most reported carbapenemases (KPC, OXA-48-like, VIM, IMP, and NDM). Direct testing of positive blood cultures could reduce time to detection. This study aims to validate and report on the diagnostic yield of a novel method for carbapenemase detection in positive blood culture vials using NG-Test CARBA 5.

METHODS

We implemented an investigator-developed method for the direct testing of positive blood cultures using NG-Test CARBA 5. We compared results between genotypic, phenotypic, and direct NG-Test CARBA 5 in blood.

RESULTS

A total of 32 isolates were tested (21 Enterobacterales and 11 ). Genotypic testing detected 23 carbapenemases. When comparing the results of NG-Test CARBA 5 in blood with genotypic testing, agreement was observed in 31/32 (97%) tests. The sensitivity, specificity, positive predictive value, and negative predictive value of the NG-Test CARBA 5 in blood were 93%, 100%, 100%, and 94%, respectively.

CONCLUSIONS

Our method using NG-Test CARBA 5 directly in blood culture samples presented an excellent diagnostic yield when compared to genotypic profiling and permits an accurate detection of carbapenemases.

摘要

背景

耐碳青霉烯类革兰氏阴性菌感染正成为医疗机构中的一项重大挑战，且在全球范围内日益受到关注。侧向流动免疫分析NG-Test CARBA 5能够检测出五种最常见的碳青霉烯酶（KPC、OXA-48样酶、VIM、IMP和NDM）。对阳性血培养物进行直接检测可缩短检测时间。本研究旨在验证并报告一种使用NG-Test CARBA 5检测阳性血培养瓶中碳青霉烯酶的新方法的诊断效能。

方法

我们采用了一种由研究人员开发的方法，使用NG-Test CARBA 5对阳性血培养物进行直接检测。我们比较了血液中基因型、表型和直接使用NG-Test CARBA 5检测的结果。

结果

共检测了32株分离菌（21株肠杆菌科细菌和11株其他细菌）。基因型检测发现了23种碳青霉烯酶。将血液中NG-Test CARBA 5的检测结果与基因型检测结果进行比较时，32次检测中有31次（97%）结果一致。血液中NG-Test CARBA 5的敏感性、特异性、阳性预测值和阴性预测值分别为93%、100%、100%和94%。

结论

与基因型分析相比，我们直接在血培养样本中使用NG-Test CARBA 5的方法具有出色的诊断效能，能够准确检测出碳青霉烯酶。