Department of Orofacial Pain and Oral Medicine, Kyung Hee University Dental Hospital, #613 Hoegi-dong, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyung Hee University, Dongdaemun-gu, Seoul 02447, Republic of Korea.
Medicina (Kaunas). 2024 Oct 25;60(11):1753. doi: 10.3390/medicina60111753.
In this study, we aimed to explore the oral bacteria and fungi that can help discern oral squamous cell carcinoma (OSCC) and investigate the correlations between multiple key pathogens. Twelve participants (8 females and 4 males; mean age, 54.33 ± 20.65 years) were prospectively recruited into three groups: Group 1: healthy control, Group 2: patients with stomatitis, and Group 3: patients with OSCC, with 4 individuals in each group. Unstimulated whole saliva samples from these participants were analyzed using real-time PCR to assess the presence and abundance of 14 major oral bacterial species and . The analysis revealed significant differences for certain microorganisms, namely, (), (), and . was most abundant in the OSCC group (5,358,692.95 ± 3,540,767.33), compared to the stomatitis (123,355.54 ± 197,490.86) and healthy control (9999.21 ± 11,998.40) groups. was undetectable in the healthy control group but was significantly more abundant in the stomatitis group (1653.94 ± 2981.98) and even higher in the OSCC group (21,336.95 ± 9258.79) ( = 0.001). A similar trend was observed for , with DNA copy numbers rising from the healthy control (464.29 ± 716.76) to the stomatitis (1861.30 ± 1206.15) to the OSCC group (9347.98 ± 5128.54) ( = 0.006). The amount of was positively correlated with (r = 0.890, < 0.001) and (r = 0.724, = 0.008). 's DNA copy number was strongly correlated with (r = 0.931, < 0.001). These three oral microbes exhibited strong positive correlations with each other and had various direct or indirect relationships with other species. In the OSCC group, , , and exhibited strong positive correlations with one another, further emphasizing the need for a deeper understanding of the complex microbial interactions in the OSCC environment.
在这项研究中，我们旨在探索有助于区分口腔鳞状细胞癌（OSCC）的口腔细菌和真菌，并研究多种关键病原体之间的相关性。 12 名参与者（8 名女性和 4 名男性；平均年龄 54.33 ± 20.65 岁）前瞻性地分为三组：组 1：健康对照组，组 2：口腔炎患者组，组 3：OSCC 患者组，每组 4 人。使用实时 PCR 分析这些参与者的未刺激全唾液样本，以评估 14 种主要口腔细菌物种和 的存在和丰度。 分析显示某些微生物存在显著差异，即 （）、 （）和 （）。 在 OSCC 组中最为丰富（5358692.95 ± 3540767.33），与口腔炎组（123355.54 ± 197490.86）和健康对照组（9999.21 ± 11998.40）相比。 在健康对照组中无法检测到，但在口腔炎组中显著更丰富（1653.94 ± 2981.98），在 OSCC 组中甚至更高（21336.95 ± 9258.79）（ = 0.001）。 对于 （）也观察到类似的趋势，DNA 拷贝数从健康对照组（464.29 ± 716.76）升高到口腔炎组（1861.30 ± 1206.15），再升高到 OSCC 组（9347.98 ± 5128.54）（ = 0.006）。 的数量与 （r = 0.890， ＜0.001）和 （r = 0.724， = 0.008）呈正相关。 的 DNA 拷贝数与 （r = 0.931， ＜0.001）呈强正相关。 这三种口腔微生物彼此之间呈强烈正相关，并与其他物种存在各种直接或间接的关系。在 OSCC 组中， 、 和 之间存在强烈的正相关，进一步强调了需要深入了解 OSCC 环境中复杂的微生物相互作用。
