Effect of Nd:YAG Laser Irradiation on the Growth of Oral Biofilm.

BACKGROUND

Oral microbiota comprises a wide variety of microorganisms. The purpose of this study was to evaluate the effects of Nd:YAG laser with a 1064 nm wavelength on the in vitro growth of , , and clinical strains, as well as their biofilm. The study also aimed to determine whether the parameters recommended for photobiomodulation (PBM) therapy, typically used for tissue wound healing, have any additional antibacterial or antifungal effects.

MATERIAL AND METHODS

Single- and dual-species planktonic cell solution and biofilm cultures of , , and were irradiated using an Nd:YAG laser (LightWalker; Fotona; Slovenia) with a flat-top Genova handpiece. Two test groups were evaluated: Group 1 (G-T1) exposed to low power associated parameters (irradiance 0.5 W/cm) and Group 2 (G-T2) with higher laser parameters (irradiance 1.75 W/cm). Group 3 (control) was not exposed to any irradiation. The lasers' effect was assessed both immediately after irradiation (DLI; Direct Laser Irradiation) and 24 h post-irradiation (24hLI) of the planktonic suspension using a quantitative method (colony-forming units per 1 mL of suspension; CFU/mL), and the results were compared with the control group, in which no laser was applied. The impact of laser irradiation on biofilm biomass was assessed immediately after laser irradiation using the crystal violet method.

RESULTS

Nd:YAG laser irradiation with photobiomodulation setting demonstrated an antimicrobial effect with the greatest immediate reduction observed in , achieving up to 85.4% reduction at the T2 settings. However, the laser's effectiveness diminished after 24 h. In single biofilm cultures, the highest reductions were noted for and at the T2 settings, with achieving a 92.6 ± 3.3% reduction and reaching a 94.3 ± 5.0% reduction. Overall, the T2 settings resulted in greater microbial reductions compared to T1, particularly in biofilm cultures, although the effectiveness varied depending on the microorganism and culture type. Laser irradiation, assessed immediately after using the crystal violet method, showed the strongest biofilm reduction for in the T2 settings for both single-species and dual-species biofilms, with higher reductions observed in all the microbial samples at the T2 laser parameters ( < 0.05) Conclusion: The Nd:YAG laser using standard parameters typically applied for wound healing and analgesic effects significantly reduced the number of ; ; and strains.