Grzech-Leśniak Zuzanna, Pyrkosz Jakub, Szwach Jagoda, Kosidło Patrycja, Matys Jacek, Wiench Rafał, Pajączkowska Magdalena, Nowicka Joanna, Dominiak Marzena, Grzech-Leśniak Kinga
Faculty of Medicine and Dentistry, Wroclaw Medical University, 50-367 Wroclaw, Poland.
Faculty of Medicine, Wroclaw Medical University, 50-367 Wroclaw, Poland.
Life (Basel). 2025 Mar 16;15(3):474. doi: 10.3390/life15030474.
In contemporary dentistry, laser-based interventions have become one of the mainstays of care for patients with oral biofilm diseases, such as candidiasis, periodontal disease and peri-implantitis. The purpose of this study was to evaluate the effectiveness of Er:YAG laser (LightWalker, Ljubljana, Fotona, Slovenia) irradiation at varying irradiance levels (T1: 11.3 W/cm and T2: 120.54 W/cm) on microbial viability in single- and dual-species biofilm models, focusing on , and , to address challenges in managing complex oral biofilms in clinically relevant settings. The results showed substantial microbial reduction, with being the most susceptible microorganism (93-99.9%), while exhibited marked resistance at higher irradiance levels. Interestingly, demonstrated varying reductions based on the biofilm composition, highlighting the influence of microbial interactions. This study concluded that the Er:YAG laser effectively reduced biofilm viability, with its efficacy depending on the microbial composition and irradiance settings. These findings highlight the need for tailored erbium laser parameters to optimize clinical outcomes, underscoring the need for individualized polymicrobial biofilm management, particularly in periodontal and peri-implant therapies.
在当代牙科领域，基于激光的干预措施已成为治疗口腔生物膜疾病（如念珠菌病、牙周病和种植体周围炎）患者的主要手段之一。本研究的目的是评估不同辐照度水平（T1：11.3 W/cm和T2：120.54 W/cm）的铒钇铝石榴石激光（LightWalker，卢布尔雅那，Fotona，斯洛文尼亚）照射对单菌种和双菌种生物膜模型中微生物活力的影响，重点关注[具体菌种1]、[具体菌种2]和[具体菌种3]，以应对在临床相关环境中管理复杂口腔生物膜的挑战。结果显示微生物大量减少，其中[最敏感的菌种]是最易受影响的微生物（减少93 - 99.9%），而[最具抗性的菌种]在较高辐照度水平下表现出明显抗性。有趣的是，[特定菌种]根据生物膜组成表现出不同程度的减少，突出了微生物相互作用的影响。本研究得出结论，铒钇铝石榴石激光有效降低了生物膜活力，其疗效取决于微生物组成和辐照度设置。这些发现凸显了需要定制铒激光参数以优化临床结果，强调了个性化管理多菌种生物膜的必要性，特别是在牙周和种植体周围治疗中。
