Suleiman Mohammed, Iqbal Muhammad, Tang Patrick, Pérez-López Andrés
Department of Pathology, Sidra Medicine, Doha P.O. Box 26999, Qatar.
Department of Pathology and Laboratory Medicine, Weill Cornell Medicine in Qatar, Doha P.O. Box 24144, Qatar.
Microorganisms. 2024 Nov 10;12(11):2282. doi: 10.3390/microorganisms12112282.
Accurate laboratory diagnosis of gastroenteritis is important to ensure that patients receive appropriate treatment and proper isolation precautions. This study evaluated the performance of the QIAGEN QIAstat-Dx gastrointestinal panel (QGP) in comparison to the bioMerieux BioFire FilmArray gastrointestinal panel (BGP) for the detection of gastrointestinal pathogens in 110 pediatric patients being evaluated for gastroenteritis at our hospital. We compared 23 different bacterial, viral, and parasite enteropathogens detected by the QGP against the BGP. The overall positive percent agreement (PPA) for all compared targets was 96.2% and the overall negative percent agreement (NPA) for all compared targets was 99.7%. Our study shows that QIAstat-Dx QGP provides comparable results to the BioFire BGP in our pediatric population. Additionally, the PCR cycle threshold (Ct) value reported by the QGP is potentially a helpful tool in estimating the load of the detected pathogen in stool samples.
准确的实验室诊断肠胃炎对于确保患者接受适当治疗和采取适当的隔离预防措施很重要。本研究评估了QIAGEN QIAstat-Dx胃肠道检测板（QGP）与bioMerieux BioFire FilmArray胃肠道检测板（BGP）在我院110名因肠胃炎接受评估的儿科患者中检测胃肠道病原体的性能。我们将QGP检测到的23种不同的细菌、病毒和寄生虫肠道病原体与BGP进行了比较。所有比较目标的总体阳性百分比一致性（PPA）为96.2%，所有比较目标的总体阴性百分比一致性（NPA）为99.7%。我们的研究表明，在我们的儿科人群中，QIAstat-Dx QGP与BioFire BGP提供了可比的结果。此外，QGP报告的PCR循环阈值（Ct）值可能是估计粪便样本中检测到的病原体载量的有用工具。
