在培养的犬细胞模型中评估益生菌混合物的生物膜形成和抗炎反应。

Assessment of Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend in a Cultured Canine Cell Model.

作者信息

Gallina Nicholas L F, Irizarry Tardi Nicole, Li Xilin, Cai Alvin, Horn Mandy J, Applegate Bruce M, Reddivari Lavanya, Bhunia Arun K

机构信息

Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907, USA.

Purdue Institute of Inflammation, Immunology, and Infectious Disease, Purdue University, West Lafayette, IN 47907, USA.

出版信息

Microorganisms. 2024 Nov 11;12(11):2284. doi: 10.3390/microorganisms12112284.

DOI:10.3390/microorganisms12112284

PMID:39597673

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11596120/

Abstract

Gut dysbiosis and an inflamed bowel are growing concerns in mammals, including dogs. Probiotic supplements have been used to restore the natural microbial community and improve gastrointestinal health. Biofilm formation, antimicrobial activities, and immunological responses of probiotics are crucial to improving gut health. Thus, we tested a commercial probiotic blend (LabMAX-3), a canine kibble additive comprising , , and for their ability to inactivate common enteric pathogens; their ability to form biofilms; epithelial cell adhesion; and their anti-inflammatory response in the Madin-Darby Canine Kidney (MDCK) cell line. Probiotic LabMAX-3 blend or individual isolates showed a strong inhibitory effect against , , enterotoxigenic , and . LabMAX-3 formed biofilms comparable to . LabMAX-3 adhesion to the MDCK cell line (with or without lipopolysaccharide (LPS) pretreatment) showed comparable adhesion and biofilm formation ( < 0.05) to ATCC 334 used as a control. LabMAX-3 had no cytotoxic effects on the MDCK cell line during 1 h exposure. The interleukin-10 (IL-10) and tumor necrosis factor alpha (TNFα) ratio of LabMAX-3, compared to the control, showed a significant increase ( < 0.05), indicating a more pronounced anti-inflammatory response. The data show that LabMAX-3, a canine kibble supplement, can improve gastrointestinal health.

摘要

肠道微生物群失调和肠道炎症在包括狗在内的哺乳动物中日益引起关注。益生菌补充剂已被用于恢复自然微生物群落并改善胃肠道健康。益生菌的生物膜形成、抗菌活性和免疫反应对于改善肠道健康至关重要。因此，我们测试了一种商业益生菌混合物（LabMAX-3），一种犬粮添加剂，其包含[具体成分1]、[具体成分2]和[具体成分3]，测试其灭活常见肠道病原体的能力；形成生物膜的能力；上皮细胞粘附能力；以及它们在麦迪逊-达比犬肾（MDCK）细胞系中的抗炎反应。益生菌LabMAX-3混合物或单个分离株对[病原体1]、[病原体2]、产肠毒素的[病原体3]和[病原体4]显示出强烈的抑制作用。LabMAX-3形成的生物膜与[对比菌株]相当。LabMAX-3对MDCK细胞系的粘附（无论有无脂多糖（LPS）预处理）与用作对照的ATCC 334相比，显示出相当的粘附和生物膜形成（P<0.05）。在1小时暴露期间，LabMAX-3对MDCK细胞系没有细胞毒性作用。与[对照物]相比，LabMAX-3的白细胞介素-10（IL-10）和肿瘤坏死因子α（TNFα）比值显著增加（P<0.05），表明其抗炎反应更为明显。数据表明，犬粮补充剂LabMAX-3可以改善胃肠道健康。