Powell J W, Stables J N, Watt R A
Mol Biochem Parasitol. 1986 Feb;18(2):171-82. doi: 10.1016/0166-6851(86)90036-8.
This study followed the metabolism of [13C]glucose under anaerobic and aerobic conditions in the adult filarial nematodes Brugia pahangi and Dipetalonema viteae using non-invasive 13C nuclear magnetic resonance techniques. Adult B. pahangi and D. viteae showed a rapid uptake of labelled glucose which remained linear over at least 4 h. Both species of worm removed significantly more glucose from the medium under aerobic conditions than under anaerobic conditions. The principal product of metabolism, under both anaerobic and aerobic conditions, was lactate, which accounted for 62-71% of the original [13C]glucose. Examination of the maintenance medium following worm incubation revealed a further excretory product which was identified as succinate. This product accounted for 1-2% of labelled glucose in adult B. pahangi and 2-5% in adult D. viteae. The presence of succinate as an excretory product suggests that a partial reversed tricarboxylic acid cycle is active in these filarial nematodes. A further peak was identified in the worm homogenate and identified as trehalose. The disaccharide was not an excretory product and occurred only within the worm. The peak accounted for 13-14% of the 13C-labelled glucose in B. pahangi and 15-16% in D. viteae. Trehalose has not been previously recorded in either of these nematodes and is likely to have a storage function.
本研究采用非侵入性的¹³C核磁共振技术，追踪了成年丝状线虫彭亨布鲁线虫（Brugia pahangi）和马来布鲁线虫（Dipetalonema viteae）在厌氧和好氧条件下[¹³C]葡萄糖的代谢情况。成年彭亨布鲁线虫和马来布鲁线虫对标记葡萄糖的摄取迅速，且至少在4小时内保持线性。在好氧条件下，这两种线虫从培养基中去除的葡萄糖比在厌氧条件下显著更多。在厌氧和好氧条件下，代谢的主要产物都是乳酸，其占原始[¹³C]葡萄糖的62 - 71%。对线虫孵育后的维持培养基进行检测，发现了另一种排泄产物，经鉴定为琥珀酸。该产物在成年彭亨布鲁线虫中占标记葡萄糖的1 - 2%，在成年马来布鲁线虫中占2 - 5%。琥珀酸作为排泄产物的存在表明，部分逆向三羧酸循环在这些丝状线虫中是活跃的。在线虫匀浆中还鉴定出了另一个峰，被确定为海藻糖。这种二糖不是排泄产物，仅存在于线虫体内。该峰在彭亨布鲁线虫中占¹³C标记葡萄糖的13 - 14%，在马来布鲁线虫中占15 - 16%。此前在这两种线虫中均未记录到海藻糖，它可能具有储存功能。
