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超声辅助提取制备的红菇纯化多糖的单糖组成、理化特性及益生元活性

Monosaccharide composition, physicochemical characteristics, and prebiotic activity of purified polysaccharides from red mushroom prepared using ultrasonic-assisted extraction.

作者信息

Chen Bo Jie, Xia Cong, Wu Peiling, Liu Yang, Li Jing, Jiang Tiemin, Li Xia, Peng Biyu, Khoo Hock Eng

机构信息

Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, Guangxi, China.

College of Biomass Science and Engineering, Sichuan University, Chengdu, Sichuan, China.

出版信息

Nat Prod Res. 2024 Nov 27:1-10. doi: 10.1080/14786419.2024.2432606.

Abstract

The objective of this study was to develop a purified polysaccharide for use in prebiotic research. The monosaccharide composition, physicochemical characteristics, and prebiotic activities of the polysaccharides were determined. Additionally, the total protein, total sugar, antioxidant activities, and molecular weights of the polysaccharides were subjected to analysis. The results demonstrated that the purified polysaccharide sample was predominantly composed of short-chain dextrans. The sample was found to comprise trehalose and trace amounts of protein. The Brunauer-Emmet-Teller (BET) analysis revealed that the polysaccharides exhibited a multiporous structure. The polysaccharide structure was found to consist of hydroxyl groups, along with the other common functional groups typically observed in sugar molecules. The polysaccharide sample exhibited moderate thermal stability, and its antioxidant activities were observed to be concentration-dependent. Furthermore, the polysaccharides were observed to facilitate the growth of probiotic strains. Therefore, the polysaccharides are potential nutraceuticals and prebiotics.

摘要

本研究的目的是开发一种用于益生元研究的纯化多糖。测定了多糖的单糖组成、理化特性和益生元活性。此外,还对多糖的总蛋白、总糖、抗氧化活性和分子量进行了分析。结果表明,纯化的多糖样品主要由短链葡聚糖组成。该样品含有海藻糖和微量蛋白质。布鲁诺尔-埃米特-泰勒(BET)分析表明,多糖呈现多孔结构。发现多糖结构由羟基以及糖分子中通常观察到的其他常见官能团组成。多糖样品表现出适度的热稳定性,并且观察到其抗氧化活性具有浓度依赖性。此外,观察到多糖促进益生菌菌株的生长。因此,这些多糖是潜在的营养保健品和益生元。

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