Oustadi Fereshteh, Stephens Emma D, Badv Maryam
Department of Biomedical Engineering, University of Calgary, Calgary, Alberta T2N 1N4, Canada.
Libin Cardiovascular Institute, University of Calgary, Calgary, Alberta T2N 4N1, Canada.
ACS Appl Mater Interfaces. 2024 Dec 11;16(49):67385-67398. doi: 10.1021/acsami.4c16080. Epub 2024 Nov 27.
In clinical settings, biofluid-contacting devices can suffer from biofouling, leading to thrombus formation and bacterial biofilm buildup, which impair device function and pose health risks. Traditional antifouling methods, including the use of hydrophilic polymers and heparin coatings, often suffer from instability and reduced bioactivity over time. Lubricant-infused surfaces (LIS) have emerged as a promising alternative due to their long-term stability and broad-spectrum repellency. However, current LIS technologies typically involve complex, multistep processes that restrict their application to surface layers, potentially compromising performance under mechanical stress. This study introduces a novel method for bulk modification of poly(vinyl alcohol) (PVA) films, creating flexible lubricant-infused PVA membranes with superior antifouling properties. These films are fabricated by cross-linking the PVA chains using n-propyltrichlorosilane (n-PTCS) and subsequent infusion with silicone oil as a lubricant. The modified PVA films significantly prevent bacterial adhesion and prolong blood and plasma clot formation. Additionally, these films exhibit enhanced mechanical properties, particularly in elasticity and flexibility compared to unmodified PVA films. The developed technique provides a straightforward method for creating flexible, super-repellent biointerfaces with the potential to prevent blood adhesion and bacterial biofilm formation, which are common complications associated with biofluid-contacting devices and medical implants.
在临床环境中，与生物流体接触的装置可能会遭受生物污染，导致血栓形成和细菌生物膜积聚，这会损害装置功能并带来健康风险。传统的防污方法，包括使用亲水性聚合物和肝素涂层，随着时间的推移往往会出现稳定性问题和生物活性降低的情况。注入润滑剂的表面（LIS）因其长期稳定性和广谱排斥性而成为一种有前景的替代方法。然而，目前的LIS技术通常涉及复杂的多步骤过程，这限制了它们仅应用于表面层，在机械应力下可能会影响性能。本研究介绍了一种对聚（乙烯醇）（PVA）薄膜进行整体改性的新方法，制备出具有优异防污性能的柔性注入润滑剂的PVA膜。这些薄膜是通过使用正丙基三氯硅烷（n-PTCS）交联PVA链并随后注入硅油作为润滑剂来制备的。改性后的PVA薄膜能显著防止细菌粘附，并延长血液和血浆凝块的形成时间。此外，与未改性的PVA薄膜相比，这些薄膜具有增强的机械性能，特别是在弹性和柔韧性方面。所开发的技术提供了一种直接的方法来创建柔性、超排斥性的生物界面，有可能防止血液粘附和细菌生物膜形成，而这些是与生物流体接触装置和医疗植入物相关的常见并发症。
