Bai Zhidong, Zhou Shun, Ullah Habib, Lu Linpeng, Chen Qian, Zuo Chao
Opt Lett. 2024 Dec 1;49(23):6861-6864. doi: 10.1364/OL.531869.
Intensity diffraction tomography (IDT) is a label-free computational microscopy technique that infers 3D refractive index (RI) and absorption distributions of objects from intensity-only measurements. Nevertheless, the inherent coherent image formation model requires sequential intensity measurements under various plane wave illuminations, resulting in time-consuming data acquisition and low imaging speed. In this Letter, we propose differential phase contrast intensity diffraction tomography (DPC-IDT), which leverages partially coherent illumination to extend the accessible spectrum range, thereby achieving high-speed, motion-free 3D tomographic microscopy. DPC-IDT integrates DPC illumination within the IDT framework, allowing 3D RI tomogram reconstruction from only four intensity images under matched asymmetric annular illumination. The effectiveness of DPC-IDT is experimentally validated by RI measurements of standard microspheres. We also demonstrate dynamic 3D imaging results of living PLC cells at a 25 Hz volume rate, highlighting its potential for high-speed biological imaging of unstained samples.
强度衍射层析成像（IDT）是一种无标记的计算显微镜技术，它仅通过强度测量来推断物体的三维折射率（RI）和吸收分布。然而，固有的相干成像模型需要在各种平面波照明下进行连续的强度测量，导致数据采集耗时且成像速度低。在本信函中，我们提出了差分相衬强度衍射层析成像（DPC-IDT），它利用部分相干照明来扩展可访问的光谱范围，从而实现高速、无运动的三维层析显微镜成像。DPC-IDT将DPC照明集成到IDT框架内，允许在匹配的非对称环形照明下仅从四个强度图像重建三维RI层析图。通过对标准微球的RI测量，实验验证了DPC-IDT的有效性。我们还展示了以25Hz的体积速率对活的PLC细胞进行动态三维成像的结果，突出了其对未染色样品进行高速生物成像的潜力。
