Department of Biochemistry, Microbiology, and Biotechnology, University of Limpopo, Private Bag X1106, Sovenga, 0727, South Africa.
Sci Rep. 2024 Nov 27;14(1):29494. doi: 10.1038/s41598-024-80877-2.
The soil microbiome is crucial for the environment and significantly impacts the ecosystem. Understanding the microbiome and its interaction with soil microorganisms is essential for improving ecological and environmental strategies. In this study, Xiphinema elongatum nematodes were collected from water berry in Sovenga Hills, Limpopo Province, South Africa, and were analyzed their associated bacterial communities using metabarcoding analysis. The findings revealed that X. elongatum forms associations with a wide range of bacterial species. Among the most abundant species identified, we found Sphingomonas sp., a bacterial species commonly found in various habitats and primarily beneficial to plants, and Candidatus Xiphinematobacter, a bacterial species commonly found in nematode species of Xiphinema as an endosymbiont. The analysis using principal component analysis (PCA) revealed that the abundance of X. elongatum in the soil is inversely correlated with clay content (r = -0.52) and soil pH levels (r = -0.98), and directly correlated with soil sand content (r = 0.88). This study provides valuable insights into the bacterial species associated with plant-parasitic nematodes in trees in South Africa. It underscores the presence of various potentially detrimental and beneficial nematode-associated bacteria. The results could potentially influence the overall quality of the soil, leading to implications for the productivity and yield of fruit crops. Additionally, the results help us understand the interaction between bacteria and X. elongatum.
土壤微生物组对环境至关重要，对生态系统有重大影响。了解微生物组及其与土壤微生物的相互作用，对于改进生态和环境策略至关重要。在这项研究中，我们从南非林波波省 Sovenga 山的水莓中采集了伸长滑刃线虫，并使用代谢组学分析对其相关的细菌群落进行了分析。研究结果表明，伸长滑刃线虫与广泛的细菌物种形成了关联。在鉴定出的最丰富的物种中，我们发现了 Sphingomonas sp.，这是一种常见于各种生境的细菌，主要对植物有益，以及 Candidatus Xiphinematobacter，这是一种常见于伸长滑刃线虫等线虫物种中的内共生菌。主成分分析 (PCA) 的分析表明，土壤中伸长滑刃线虫的丰度与粘土含量 (r = -0.52) 和土壤 pH 值 (r = -0.98) 呈负相关，与土壤砂含量 (r = 0.88) 呈正相关。这项研究提供了有关南非树木中与植物寄生线虫相关的细菌物种的有价值的见解。它强调了存在各种潜在有害和有益的线虫相关细菌。研究结果可能会影响土壤的整体质量，从而对水果作物的生产力和产量产生影响。此外，研究结果有助于我们了解细菌与伸长滑刃线虫之间的相互作用。
