Biology Division, Veterinary Research Institute, Ministry of Agriculture, Tamsui, New Taipei City, Taiwan.
Department of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
Sci Rep. 2024 Nov 27;14(1):29459. doi: 10.1038/s41598-024-80943-9.
A total of 297 Escherichia coli isolates from diseased livestock with diarrhea and 269 isolates from poultry affected by colibacillosis were assessed for extended-spectrum-β-lactamase (ESBL) production. In livestock, 36 isolates (12.1%) were ESBL-producing, with pigs (52.8%), cattle (30.5%), and goats (16.7%) being the most affected. Poultry exhibited 22 ESBL-producing strains (8.6%), with distribution among species: chicken (36.3%), duck (22.7%), goose (22.7%), and others (18.2%). ESBL-producing E. coli demonstrated higher drug resistance, except for amoxicillin/clavulanic acid, while all isolates were susceptible to imipenem. The bla gene, from the bla group, was prevalent in the ESBL-producing E. coli from livestock and poultry. Multilocus sequence typing (MLST) identified distinct sequence types (STs) for 58 ESBL-producing E. coli, except for ST162 and ST1196, detected in both sources. Livestock yielded one ST10 and two ST38 isolates, while poultry exhibited two ST69 and one ST617 isolates, recognized as common extraintestinal pathogenic E. coli (ExPEC) types. In conjugation assays, all ESBL-producing E. coli successfully transferred bla genes to the recipient E. coli J53 strain. The findings underscore food-producing animals as significant ESBL reservoirs, emphasizing the crucial role of judicious antimicrobial use on farms.
从患有腹泻的病畜和患有大肠杆菌病的家禽中，共评估了 297 株产超广谱β-内酰胺酶（ESBL）的大肠杆菌和 269 株产 ESBL 的大肠杆菌。在牲畜中，有 36 株（12.1%）为产 ESBL 株，受影响最大的是猪（52.8%）、牛（30.5%）和山羊（16.7%）。家禽中有 22 株（8.6%）产 ESBL 株，分布在不同的物种中：鸡（36.3%）、鸭（22.7%）、鹅（22.7%）和其他（18.2%）。产 ESBL 的大肠杆菌表现出更高的耐药性，除了阿莫西林/克拉维酸，而所有分离株对亚胺培南均敏感。bla 基因，来自 bla 组，在来自牲畜和家禽的产 ESBL 的大肠杆菌中普遍存在。多位点序列分型（MLST）确定了 58 株产 ESBL 的大肠杆菌的不同序列类型（ST），除了在两个来源中都检测到的 ST162 和 ST1196。牲畜中产生了一株 ST10 和两株 ST38 分离株，而家禽中则产生了两株 ST69 和一株 ST617 分离株，这些分离株被认为是常见的肠外致病性大肠杆菌（ExPEC）类型。在接合试验中，所有产 ESBL 的大肠杆菌都成功地将 bla 基因转移到了受体大肠杆菌 J53 株中。这些发现强调了食用动物作为重要的 ESBL 储库的作用，强调了在农场中合理使用抗生素的关键作用。
