破除RecBCD核酸酶会破坏噬菌体的教条。

Debunking the dogma that RecBCD nuclease destroys phage.

作者信息

Zheng Clarence, Furukawa Curtis, Liu Jerry, Sankaran Srishti, Lin Han, Munugeti Nidhi, Wang Meranda, Smith Gerald R

机构信息

Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

University of California, San Diego, La Jolla, CA 92093, USA.

出版信息

Genetics. 2025 Jul 9;230(3). doi: 10.1093/genetics/iyae199.

DOI:10.1093/genetics/iyae199

PMID:39605281

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12239210/

Abstract

For decades, it has been repeatedly claimed that the potent bacterial helicase-nuclease RecBCD (exonuclease V) destroys foreign (nonself) DNA, such as that of phages, but repairs and recombines cellular (self) DNA. While this would constitute a strong host-survival mechanism, no phage destroyed by RecBCD is ever specified in those claims. To determine which phages are destroyed by RecBCD, we searched for phage isolates that grow on Escherichia coli ΔrecBCD but not on recBCD+. In contrast to the prevailing claim, we found none among >80 new isolates from nature and >80 from previous collections. Based on these and previous observations, we conclude that RecBCD repairs broken DNA that can recombine but destroys DNA that cannot recombine and recycles the nucleotides.

摘要

几十年来，一直有人反复声称，强大的细菌解旋酶核酸酶RecBCD（外切核酸酶V）会破坏外来（非自身）DNA，如噬菌体的DNA，但能修复细胞（自身）DNA并使其重组。虽然这将构成一种强大的宿主生存机制，但在这些说法中，从未明确指出有哪种噬菌体是被RecBCD破坏的。为了确定哪些噬菌体被RecBCD破坏，我们寻找了能在大肠杆菌ΔrecBCD上生长但不能在recBCD+上生长的噬菌体分离株。与普遍的说法相反，我们在来自自然界的80多个新分离株和之前收集的80多个分离株中均未找到。基于这些以及之前的观察结果，我们得出结论，RecBCD会修复能够重组的断裂DNA，但会破坏无法重组的DNA，并使核苷酸循环利用。