Lee Brandon H, Yang Zhiyuan, Ho Tiffany, Wang Yongmei, Tamura Nobumichi, Webb Samuel, Bone Sharon, Ho Sunita P
Preventive and Restorative Dent. Sci., San Francisco, CA.
Neuroscience Graduate Group, University of California, Davis, Davis, CA.
bioRxiv. 2024 Nov 14:2024.11.11.623079. doi: 10.1101/2024.11.11.623079.
In this study, we performed synchrotron-based micro-X-ray fluorescence (μ-XRF) imaging of elements Zn and S, and X-ray absorption near edge spectroscopy (XANES) coupled with μ-XRF for identification of Zn and S species in the condylar zones of a rat temporomandibular joint (TMJ). Histologic localization of Zn and hypoxia-inducible factor-1α (HIF-1α) were mapped using an optical microscope. These data were visually correlated with μ-XRF and XANES data to provide insights into plausible biological S-species in Z-enriched condylar zones of a rat TMJ. Furthermore, μ-XRF coupled with micro-X-ray diffraction (μ-XRD) was used to underline Z-incorporated biological apatite in the subchondral bone and bone of the rat TMJ. Results illustrated the potential dependence between biometal Zn and nonmetal S and their collective governance of cell and tissue functions in a zone-specific manner. Elemental Zn with organic and inorganic S-species at the cartilage-bone interface and transformation of plausible Zn-enriched mineralization kinetics of biological apatite from subchondral bone to condylar bone were ascertained using μ-XRF-XANES and μ-XRD. The coupled μ-XRF-XANES complementing with μ-XRD and immunohistology provided an informative view of S and Zn and their association with zone-specific biological pathways . Understanding the spatial distributions of the main S-species with redox-inert Zn in regions of cartilage, bone, and the interface is essential for further unlocking questions surrounding formation and resorption-related biomineralization pathways as related to osteoarthritis or genetically inherited diseases. Using these complementary techniques with microspectroscopic spatial information provided insights into the associations between biometal Zn and nonmetal S and a window into detecting the plausible early-stage diagnostic biomarkers for humans with TMJ osteoarthritis.
在本研究中，我们对大鼠颞下颌关节（TMJ）髁突区的锌（Zn）和硫（S）元素进行了基于同步辐射的微X射线荧光（μ-XRF）成像，并结合μ-XRF进行X射线吸收近边光谱（XANES）分析，以鉴定Zn和S的物种。使用光学显微镜绘制了Zn和缺氧诱导因子-1α（HIF-1α）的组织学定位图。这些数据与μ-XRF和XANES数据进行了直观关联，以深入了解大鼠TMJ富含Zn的髁突区可能存在的生物S物种。此外，μ-XRF与微X射线衍射（μ-XRD）相结合，用于强调大鼠TMJ软骨下骨和骨中含Zn的生物磷灰石。结果表明生物金属Zn和非金属S之间可能存在依赖性，以及它们以区域特异性方式对细胞和组织功能的共同调控。利用μ-XRF-XANES和μ-XRD确定了软骨-骨界面处含有有机和无机S物种的元素Zn，以及从软骨下骨到髁突骨的富含Zn的生物磷灰石矿化动力学的可能转变。μ-XRF-XANES与μ-XRD和免疫组织学相结合，提供了关于S和Zn及其与区域特异性生物途径关联的信息性视图。了解氧化还原惰性Zn在软骨、骨和界面区域中主要S物种的空间分布，对于进一步解开与骨关节炎或遗传遗传性疾病相关的形成和吸收相关生物矿化途径周围的问题至关重要。使用这些具有微观光谱空间信息的互补技术，深入了解了生物金属Zn和非金属S之间的关联，并为检测TMJ骨关节炎患者可能的早期诊断生物标志物提供了一个窗口。
