Elliott Matthew A T, Andrews John P, van der Molen Tjitse, Geng Jinghui, Spaeth Alex, Toledo Anna, Voitiuk Kateryna, Core Cordero, Gillespie Thomas, Sinervo Ari, Parks David F, Robbins Ash, Solís Daniel, Chang Edward F, Nowakowski Tomasz Jan, Teodorescu Mircea, Haussler David, Sharf Tal
Department of Biomolecular Engineering, University of California Santa Cruz, Santa Cruz, CA, USA.
UC Santa Cruz Genomics Institute, University of California Santa Cruz, Santa Cruz, CA, USA.
bioRxiv. 2025 Mar 18:2024.11.13.623525. doi: 10.1101/2024.11.13.623525.
How seizures begin at the level of microscopic neuronal circuits remains unknown. Advancements in high-density CMOS-based microelectrode arrays can be harnessed to study neuronal network activity with unprecedented spatial and temporal resolution. We use high-density electrophysiology recordings to probe the network activity of human hippocampal brain slices from six patients with mesial temporal lobe epilepsy. Two slices from the dentate gyrus exhibited epileptiform activity in the presence of low magnesium media with kainic acid. Both slices exhibit network oscillations indicative of a reciprocally connected circuit, which is unexpected under normal physiological conditions. Future studies may apply this approach to elucidate the network signals that underlie seizure initiation.
癫痫发作在微观神经元回路层面是如何开始的仍然未知。基于高密度互补金属氧化物半导体的微电极阵列技术的进步，可用于以前所未有的空间和时间分辨率研究神经元网络活动。我们使用高密度电生理记录来探测6例内侧颞叶癫痫患者的人海马脑片的网络活动。在含有红藻氨酸的低镁培养基中，来自齿状回的两片脑片表现出癫痫样活动。两片脑片均表现出指示相互连接回路的网络振荡，这在正常生理条件下是出乎意料的。未来的研究可能会应用这种方法来阐明癫痫发作起始背后的网络信号。
