Chen Shuyu, Chen YuYi, Zhang Mengyan, Zhang Wenyu, Fu Huanru, Huang Yu, Cheng Longfei, Wan Chunhe
Fujian Key Laboratory for Avian Diseases Control and Prevention, Fujian Academy of Agricultural Sciences, Institute of Animal Husbandry and Veterinary Medicine, Fujian Animal Diseases Control Technology Development Centre, Fuzhou, China.
School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
Front Vet Sci. 2024 Nov 13;11:1483990. doi: 10.3389/fvets.2024.1483990. eCollection 2024.
Duck adeno-associated Virus (DAAV) is a novel pathogen that was recently discovered in ducks. To establish a molecular detection assay for DAAV for further epidemiological investigation and pathogenic mechanism. Here, we designed specific primers and probes according to the sequence characteristics of the newly discovered DAAV and then established a TaqMan real-time PCR method (TaqMan-qPCR) for the detection of DAAV. Our data showed that the established TaqMan-qPCR for detecting DAAV had high sensitivity, with the lowest detection limit of 29.1 copies/μL. No cross reaction was found with duck circovirus (DuCV), H9N2 subtype avian influenza virus (AIV), avian Tembusu virus (ATmV). duck hepatitis A virus 1 and 3 (DHAV-1 and DHAV-3), duck adenovirus A (DAdV-A), duck adenovirus 3 (DAdV-3), or duck enteritis virus (DEV). The repeatability was excellent, with the coefficients of variation of repeated intragroup and intergroup tests ranging from 0.12-0.21% and 0.62-1.42%, respectively. Seventy-eight clinical samples collected from diseased or deceased ducklings were tested. The results showed that the DAAV positive rate was 21.79%, and a triple infection (DAAV+MDPV+GPV) was found. These data provide technical support for further molecular epidemiological surveillance and pathogenic mechanism studies of DAAV infection.
鸭腺相关病毒（DAAV）是一种最近在鸭中发现的新型病原体。为建立用于DAAV的分子检测方法以进行进一步的流行病学调查和致病机制研究。在此，我们根据新发现的DAAV的序列特征设计了特异性引物和探针，然后建立了用于检测DAAV的TaqMan实时荧光定量PCR方法（TaqMan-qPCR）。我们的数据表明，所建立的用于检测DAAV的TaqMan-qPCR具有高灵敏度，最低检测限为29.1拷贝/μL。与鸭圆环病毒（DuCV）、H9N2亚型禽流感病毒（AIV）、禽坦布苏病毒（ATmV）、鸭甲型肝炎病毒1型和3型（DHAV-1和DHAV-3）、鸭腺病毒A（DAdV-A）、鸭腺病毒3型（DAdV-3）或鸭肠炎病毒（DEV）均未发现交叉反应。重复性极佳，组内和组间重复试验的变异系数分别为0.12 - 0.21%和0.62 - 1.42%。对从患病或死亡雏鸭收集的78份临床样本进行了检测。结果显示，DAAV阳性率为21.79%，并且发现了三重感染（DAAV + MDPV + GPV）。这些数据为进一步开展DAAV感染的分子流行病学监测和致病机制研究提供了技术支持。
