Dunsmuir P, Smith S, Bedbrook J
Nucleic Acids Res. 1983 Jun 25;11(12):4177-83. doi: 10.1093/nar/11.12.4177.
The sequences in the petunia genome which encode the small subunit polypeptides of the chloroplast enzyme ribulose-1,5-bisphosphate carboxylase have been characterized. Sequence analysis of four cDNA clones indicate that there are several distinct genes transcribed in leaf tissue. There is 8-9% nucleotide divergence between the transcripts however these changes do not alter the encoded amino acid sequence. Examination of nuclear DNA by Southern hybridization and analysis of cloned small subunit genes confirm that there are a number of different genes which encode this single protein.
已对矮牵牛基因组中编码叶绿体酶核酮糖-1,5-二磷酸羧化酶小亚基多肽的序列进行了表征。对四个cDNA克隆的序列分析表明,叶组织中有几个不同的基因被转录。转录本之间存在8-9%的核苷酸差异,然而这些变化并未改变编码的氨基酸序列。通过Southern杂交对核DNA进行检测以及对克隆的小亚基基因进行分析,证实存在许多编码这种单一蛋白质的不同基因。