Grupo de Pesquisa, Ensino e Extensão em Produtos Naturais na Clínica Médica Veterinária, Departamento de Clínicas Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas - UFPel, Pelotas, RS, Brasil.
Projeto Dioctophyme renale em Cães e Gatos, Departamento de Clínicas Veterinária, Faculdade de Veterinária, Universidade Federal de Pelotas - UFPel, Pelotas, RS, Brasil.
Rev Bras Parasitol Vet. 2024 Nov 22;33(4):e016224. doi: 10.1590/S1984-29612024067. eCollection 2024.
Dioctophyme renale is a zoonotic nematode that parasitizes mainly right kidney of domestic and wild canines, and can affect humans, and its eggs are eliminated in urine. The duration of egg dissemination after surgical treatment is unknown. Therefore, the objective of this study was to identify, quantify, and verify the duration of the elimination of D. renale eggs in dog urine after the surgical removal of nematodes. The study involved 15 dogs in which female specimens of D. renale were detected in kidneys. Urine samples, preferably first-morning urine samples, were collected before and for the first ten days after nephrectomy. For egg quantification, 40 µL samples of urinary sediment were analyzed in triplicate. In laboratory analyses, between 900 and > 6,000 eggs/urine sample were detected in 86.7% of the dogs prior to surgery, and in 40% of the dogs on postoperative day 1. Of the 15 dogs evaluated, 14 (93.3%) eliminated D. renale eggs on each of the first ten postoperative days. Egg elimination peaked on postoperative day 1. Our results indicate that dogs can continue to be sources of D. renale infection even after the helminths have been removed from a parasitized kidney, underscoring the novelty of these findings and their importance for the One Health approach.
肾膨结线虫是一种寄生在犬科动物右肾的人畜共患线虫，可感染人类，其虫卵随尿液排出。手术治疗后虫卵传播的持续时间尚不清楚。因此，本研究的目的是确定、定量和验证犬肾膨结线虫手术后犬尿液中肾膨结线虫虫卵的消除时间。该研究涉及 15 只犬，这些犬的肾脏中检测到雌性肾膨结线虫标本。在肾切除术之前和之后的前十天内，采集尿液样本，最好是晨尿。为了进行卵定量分析，对 40 µL 尿液沉淀物样本进行了三次重复分析。在实验室分析中，在手术前，86.7%的犬尿液样本中检测到 900 至> 6000 个/尿液样本，在术后第 1 天，40%的犬尿液样本中检测到。在评估的 15 只犬中，有 14 只（93.3%）在术后的前十天内每天都排出肾膨结线虫虫卵。卵的排泄在术后第 1 天达到高峰。我们的结果表明，即使寄生虫已从受感染的肾脏中移除，犬仍可继续成为肾膨结线虫感染的来源，这突显了这些发现的新颖性及其对“同一健康”方法的重要性。
