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大鼠rRNA转录单元侧翼间隔区的核苷酸序列分析及重复元件的鉴定。

Nucleotide sequence analysis of the spacer regions flanking the rat rRNA transcription unit and identification of repetitive elements.

作者信息

Yavachev L P, Georgiev O I, Braga E A, Avdonina T A, Bogomolova A E, Zhurkin V B, Nosikov V V, Hadjiolov A A

出版信息

Nucleic Acids Res. 1986 Mar 25;14(6):2799-810. doi: 10.1093/nar/14.6.2799.

DOI:10.1093/nar/14.6.2799
PMID:3960734
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339699/
Abstract

We investigated the organization of the rat rDNA non-transcribed spacer (NTS) by determining the sequence of large NTS segments located upstream (2501 bp) and downstream (4025 bp) from the rRNA transcription unit. We identified four B2-like and two ID mobile elements. They may be grouped in three pairs with the members of each pair located in the upstream and downstream NTS. The ID sequences are identical to the consensus sequence, while the pairs of B2-like elements show 85% and 50/65% homology to the consensus B2 sequence. The proximal part of the downstream NTS contains a region of widely diverged SalI tandem repeats. A considerable part of the analyzed upstream and downstream NTS sequences is constituted by different types of simple sequences and long poly(purine) X poly(pyrimidine) tracts. These data show that the rat rDNA NTS regions flanking the rRNA transcription unit are characterized by a combination of short interspersed (B2-superfamily) and various simple sequences.

摘要

我们通过测定位于rRNA转录单元上游（2501 bp）和下游（4025 bp）的大的非转录间隔区（NTS）片段的序列，研究了大鼠rDNA非转录间隔区的组织情况。我们鉴定出四个B2样元件和两个ID移动元件。它们可以分成三对，每对的成员分别位于上游和下游NTS中。ID序列与共有序列相同，而B2样元件对与共有B2序列的同源性分别为85%和50/65%。下游NTS的近端部分包含一个差异很大的SalI串联重复区域。分析的上游和下游NTS序列的相当一部分由不同类型的简单序列和长的聚嘌呤X聚嘧啶片段组成。这些数据表明，rRNA转录单元两侧的大鼠rDNA NTS区域的特征是短散在元件（B2超家族）和各种简单序列的组合。

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