Arnheim N, Seperack P, Banerji J, Lang R B, Miesfeld R, Marcu K B
Cell. 1980 Nov;22(1 Pt 1):179-85. doi: 10.1016/0092-8674(80)90166-x.
When a cloned 6 kb Eco RI-Sal I fragement of mouse ribosomal gene nontranscribed spacer DNA (rDNA NTS) was used to screen a BALB/c mouse gene library, 25% of the recombinant phage hybridized with it. In situ hybridization experiments and characterization of 12 clones selected using this probe supported the idea that sequences homologous to this rDNA NTS region are scattered throughout the genome. Subsequently, sequences homologous to mouse rDNA NTS were found flanking mouse mu, alpha and gamma 2b immunoglobulin CH genes. One region was localized 3' to the mu coding sequence, an area which has been identified as an intervening sequence between the secreted C mu heavy chain terminus and the C terminal portion of the membrane-bound C mu heavy chain.
当用克隆的6 kb小鼠核糖体基因非转录间隔区DNA（rDNA NTS）的Eco RI-Sal I片段筛选BALB/c小鼠基因文库时，25%的重组噬菌体与之杂交。使用该探针选择的12个克隆的原位杂交实验和特性分析支持了这样一种观点，即与该rDNA NTS区域同源的序列分散在整个基因组中。随后，在小鼠μ、α和γ2b免疫球蛋白CH基因两侧发现了与小鼠rDNA NTS同源的序列。一个区域定位在μ编码序列的3'端，该区域已被确定为分泌型Cμ重链末端与膜结合型Cμ重链C末端部分之间的间隔序列。
