Lignin, a vital plant component, is key in providing structural integrity and is the second most abundant biopolymer in nature. The growing interest in sustainable and efficient biocatalysis has driven the exploration of lignin nanoparticles (LNPs) as a promising platform for enzyme immobilization. Given lignin's abundance and structural role in plants, converting it into nanoparticles offers a potential eco-friendly alternative to traditional supports. This comprehensive review explores recent advancements in using LNPs for enzyme immobilization, focusing on loading techniques, immobilization efficiency, enzyme activity levels, and various factors that affect the performance of enzymes immobilized on LNPs. The review also addresses the primary challenges associated with enzyme immobilization on LNPs and discusses future innovations in this field. Adopting eco-friendly immobilization platforms based on LNPs is expected to have broad applications in industries like food, pharmaceuticals, animal feed, and detergents. However, there is still potential to customize LNPs further and develop novel immobilization techniques to leverage their benefits fully. By understanding the properties and advantages of these nanostructured lignin supports, researchers can design and create innovative nanocatalysts for various industrial applications.