Gorish Babbiker Mohammed Taher, Abdelmula Waha Ismail Yahia, Sethupathy Sivasamy, Robele Ashenafi Berhanu, Zhu Daochen
International Joint Laboratory on Synthetic Biology and Biomass Biorefinery, Biofuels Institute, School of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, PR China.
Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou, 215009, China.
Appl Biochem Biotechnol. 2025 Mar;197(3):1393-1418. doi: 10.1007/s12010-024-05133-9. Epub 2024 Nov 28.
Lignin, a vital plant component, is key in providing structural integrity and is the second most abundant biopolymer in nature. The growing interest in sustainable and efficient biocatalysis has driven the exploration of lignin nanoparticles (LNPs) as a promising platform for enzyme immobilization. Given lignin's abundance and structural role in plants, converting it into nanoparticles offers a potential eco-friendly alternative to traditional supports. This comprehensive review explores recent advancements in using LNPs for enzyme immobilization, focusing on loading techniques, immobilization efficiency, enzyme activity levels, and various factors that affect the performance of enzymes immobilized on LNPs. The review also addresses the primary challenges associated with enzyme immobilization on LNPs and discusses future innovations in this field. Adopting eco-friendly immobilization platforms based on LNPs is expected to have broad applications in industries like food, pharmaceuticals, animal feed, and detergents. However, there is still potential to customize LNPs further and develop novel immobilization techniques to leverage their benefits fully. By understanding the properties and advantages of these nanostructured lignin supports, researchers can design and create innovative nanocatalysts for various industrial applications.
木质素是植物的重要组成部分，对于提供结构完整性至关重要，并且是自然界中第二丰富的生物聚合物。对可持续和高效生物催化的兴趣日益浓厚，推动了对木质素纳米颗粒（LNPs）作为酶固定化有前景平台的探索。鉴于木质素在植物中的丰富性及其结构作用，将其转化为纳米颗粒为传统载体提供了一种潜在的环保替代方案。这篇综述探讨了利用LNPs进行酶固定化的最新进展，重点关注负载技术、固定化效率、酶活性水平以及影响固定在LNPs上的酶性能的各种因素。该综述还阐述了与在LNPs上固定酶相关的主要挑战，并讨论了该领域未来的创新。采用基于LNPs的环保固定化平台有望在食品、制药、动物饲料和洗涤剂等行业得到广泛应用。然而，进一步定制LNPs并开发新型固定化技术以充分利用其优势仍有潜力。通过了解这些纳米结构木质素载体的特性和优势，研究人员可以设计和创建用于各种工业应用的创新型纳米催化剂。
