Li Guiyin, Li Wenzhan, Zhou Yu, Tan Xiaohong, Huang Qing, Liang Jintao, Zhou Zhide
College of Chemistry, Guangdong University of Petrochemical Technology, Guandu Road, Maoming, Guangdong 525000, PR China.
School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi 541004, PR China.
Bioelectrochemistry. 2025 Apr;162:108855. doi: 10.1016/j.bioelechem.2024.108855. Epub 2024 Nov 27.
Glypican-3 (GPC3) is exclusively overexpressed in most Hepatocellular carcinoma (HCC) tissue but not in normal liver tissue, making it a promising biomarker for the precise detection of HCC. In this paper, a label-free light-addressable potentiometric sensor (LAPS) decorated by platinumpalladium-hemin-reduced graphene oxide nanocomposites (PtPd@H-rGO NCs) was constructed for determination of GPC3. The GPC3 aptamer (GPC3) and PtPd@H-rGO NCs were modified on the surface of silicon-based LAPS chip to build sensitive unit of LAPS system. A readout photocurrent elicited from a modulated light source, registers the localized surface potential change. When a bias voltage is provided to the LAPS system, the GPC3-GPC3 complexes formed by the specific reaction between GPC3 and GPC3 at the sensing interface can cause the sensitive membrane surface potential to change, resulting in the photocurrent-voltage (I-V) curves generate a corresponding offset response. Therefore GPC3 concentration can be determined by monitoring the potential shifts (△V). Under optimal conditions, the potential shift is linearly related to the concentration of GPC3 in the range of 0.001-3.00 μg/mL with the limit of detection (LOD) of 0.0001 μg/mL. The LAPS has a good analytical performance with good specificity, reproducibility and stability, and can be used for the detection of GPC3 in actual serum samples, which provides a broad application prospect for the combined application of LAPS and aptamers in biooassay.
磷脂酰肌醇蛋白聚糖-3（GPC3）仅在大多数肝细胞癌（HCC）组织中过度表达，而在正常肝组织中不表达，这使其成为精确检测HCC的有前景的生物标志物。本文构建了一种由铂钯-血红素-还原氧化石墨烯纳米复合材料（PtPd@H-rGO NCs）修饰的无标记光寻址电位传感器（LAPS），用于测定GPC3。将GPC3适配体（GPC3）和PtPd@H-rGO NCs修饰在硅基LAPS芯片表面，构建LAPS系统的敏感单元。由调制光源引出的读出光电流记录局部表面电位变化。当向LAPS系统提供偏置电压时，传感界面处GPC3与GPC3之间的特异性反应形成的GPC3-GPC3复合物可导致敏感膜表面电位发生变化，从而使光电流-电压（I-V）曲线产生相应的偏移响应。因此，可通过监测电位变化（△V）来测定GPC3浓度。在最佳条件下，电位变化与GPC3浓度在0.001-3.00μg/mL范围内呈线性关系，检测限（LOD）为0.0001μg/mL。该LAPS具有良好的分析性能，特异性、重现性和稳定性良好，可用于实际血清样品中GPC3的检测，为LAPS与适配体在生物分析中的联合应用提供了广阔的应用前景。
