Liang Shaojie, Wang Kun, Mao Dabin, Ouyang Qianqian, Lv Xiaoping, Xie Liwei, Zhu Dajian
Maternal and Children's Health Research Institute, Shunde Maternal and Children's Hospital, Guangdong Medical University, Foshan 528300, China; The Marine Biomedical Research Institute of Guangdong Zhanjiang, Zhanjiang 524023, China.
Maternal and Children's Health Research Institute, Shunde Maternal and Children's Hospital, Guangdong Medical University, Foshan 528300, China.
Toxicol Appl Pharmacol. 2025 Jan;494:117175. doi: 10.1016/j.taap.2024.117175. Epub 2024 Nov 26.
In this study, we investigated the regulatory role of curcumin in the differentiation of intestinal stem cells (ISCs) in dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) model mice and explored whether this effect was mediated by the Wnt/β-catenin signaling pathway. We conducted experiments in DSS-induced UC model mice to observe changes in intestinal morphology through HE staining and detect the expression of key proteins in the Wnt/β-catenin signaling pathway. According to these findings, curcumin was found to have a significant impact on the differentiation of ISCs. These results indicated that curcumin inhibited the Wnt/β-catenin signaling pathway and restored ISC differentiation. The effects of curcumin on the Wnt/β-catenin signaling pathway were further confirmed using Wnt/β-catenin agonists. These findings provide a new perspective for understanding the behavior of ISCs in the context of inflammation and offer new insights into the development of novel therapeutic strategies and drugs for UC.
在本研究中，我们调查了姜黄素在葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）模型小鼠肠道干细胞（ISC）分化中的调节作用，并探讨了这种作用是否由Wnt/β-连环蛋白信号通路介导。我们在DSS诱导的UC模型小鼠中进行实验，通过苏木精-伊红（HE）染色观察肠道形态变化，并检测Wnt/β-连环蛋白信号通路中关键蛋白的表达。根据这些发现，发现姜黄素对ISC的分化有显著影响。这些结果表明，姜黄素抑制Wnt/β-连环蛋白信号通路并恢复ISC分化。使用Wnt/β-连环蛋白激动剂进一步证实了姜黄素对Wnt/β-连环蛋白信号通路的影响。这些发现为理解炎症背景下ISC的行为提供了新的视角，并为UC新型治疗策略和药物的开发提供了新的见解。
