Department of Veterinary Surgery, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Konkuk University, Seoul 05029, Korea.
J Vet Sci. 2024 Nov;25(6):e80. doi: 10.4142/jvs.24163.
The intravenous administration of adipose tissue-derived mesenchymal stem cells (AdMSCs) in veterinary medicine is a promising regenerative therapy, but it can lead to severe complications, including pulmonary thromboembolism (PTE).
As part of an ongoing study, this study examined the impact of medications, such as heparin, aspirin, and sodium nitroprusside (SNP), on the factors linked to PTE after an intravenous injection of canine mesenchymal stem cell into experimental animals.
Fluorescently labeled canine AdMSCs were administered intravenously into the tail veins of five-week-old male BALB/c hairless mice. This study compared the survival rates, biodistribution, platelet counts, D-dimer levels, and histological examination results among the drug treatment experimental and the control groups.
The final survival rates in the SNP, control aspirin, and heparin groups were 25%, 33%, 50%, and 100%, respectively. imaging confirmed fluorescence exclusively in the lungs of all subjects who died during the injection, with no fluorescence detected in the other organs. On the other hand, in the heparin experimental group, the surviving individuals exhibited fluorescence in the lungs and the liver on day one. Histological biopsies revealed PTE in all deceased individuals within the medication experimental groups ( = 0.029).
Heparin was highly effective, with no PTE-related deaths observed when used alongside cell injections. Aspirin revealed moderate effectiveness, surpassing the control group. On the other hand, the efficacy of SNP was inferior to that of the other two drugs.
在兽医医学中，静脉内给予脂肪组织源性间充质干细胞（AdMSCs）是一种很有前途的再生治疗方法，但它会导致严重的并发症，包括肺血栓栓塞症（PTE）。
作为正在进行的研究的一部分，本研究检查了肝素、阿司匹林和硝普钠（SNP）等药物对静脉内注射犬间充质干细胞后与 PTE 相关的因素的影响。
将荧光标记的犬 AdMSCs 静脉内注射到 5 周龄雄性 BALB/c 无毛小鼠的尾静脉中。本研究比较了药物治疗实验组和对照组的存活率、生物分布、血小板计数、D-二聚体水平和组织学检查结果。
SNP、对照阿司匹林和肝素组的最终存活率分别为 25%、33%、50%和 100%。成像确认了所有在注射过程中死亡的动物的肺部仅存在荧光，而其他器官均未检测到荧光。另一方面，在肝素实验组中，存活的个体在注射后第一天肺部和肝脏均显示荧光。组织学活检显示在药物实验组的所有死亡个体中均存在 PTE（ = 0.029）。
肝素与细胞注射一起使用时非常有效，没有观察到与 PTE 相关的死亡。阿司匹林显示出中等疗效，超过了对照组。另一方面，SNP 的疗效不如其他两种药物。
