Park Sang Ryeol, Son Seungmin
National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, Republic of Korea.
Front Plant Sci. 2024 Nov 14;15:1501092. doi: 10.3389/fpls.2024.1501092. eCollection 2024.
The environmental conditions play a crucial role in determining crop yield, which is essential for ensuring food and nutritional security. However, rapid climate change is exacerbating global environmental stress, leading to severe biotic pressures on crops. Therefore, enhancing crop resilience to pathogens has become one of the most pressing challenges for humanity. Large-scale mutant library screening is the most efficient strategy for identifying numerous genes associated with specific traits. The revolutionary CRISPR/Cas9 system has ushered in a new era in the construction of mutant library. However, its application in crop plants has been relatively scarce compared to mammals, largely due to challenges in accessibility. Fortunately, several research groups have recently developed CRISPR/Cas9-based mutant libraries, successfully identifying a variety of genes involved in crop immunity. In this review, we present an overview and discussion of studies that have generated significant results through the use of CRISPR/Cas9 library screening to identify novel genes associated with resistance to biotic stresses within the field of plant research.
环境条件在决定作物产量方面起着至关重要的作用，而作物产量对于确保粮食和营养安全至关重要。然而，快速的气候变化正在加剧全球环境压力，给作物带来严重的生物压力。因此，提高作物对病原体的抗性已成为人类面临的最紧迫挑战之一。大规模突变体文库筛选是鉴定与特定性状相关的众多基因的最有效策略。革命性的CRISPR/Cas9系统开创了突变体文库构建的新时代。然而，与哺乳动物相比，其在作物植物中的应用相对较少，这主要是由于可及性方面的挑战。幸运的是，最近有几个研究小组开发了基于CRISPR/Cas9的突变体文库，成功鉴定出了多种参与作物免疫的基因。在这篇综述中，我们概述并讨论了通过使用CRISPR/Cas9文库筛选在植物研究领域中鉴定与生物胁迫抗性相关的新基因而取得显著成果的研究。
