Liu Ruilin, He Tong, Li Ruizhi, Wang Shiwei, Lai Chen, Zhang Kun
Aesthet Surg J Open Forum. 2024 Oct 22;6:ojae091. doi: 10.1093/asjof/ojae091. eCollection 2024.
Biodegradable polymers are commonly used as dermal fillers in plastic surgery. Among these, poly-L-lactic acid (PLLA) distinguishes itself owing to its good biocompatibility, degradability, and ability to act as a collagen stimulator.
In this study, the differential behavior of PLLA microspheres with varying microscopic morphology and surface hydrophilicity was investigated both in vitro and in vivo.
The introduction of short hydrophilic polyethylene glycol (PEG) chains into the PLLA molecule was employed to modify the morphology and enhance the surface hydrophilicity of the microspheres. The morphology and physicochemical properties of the PLLA and PLLA-b-PEG microspheres were characterized. Irregular PLLA particles, PLLA, and PLLA-b-PEG microspheres were implanted into the subcutaneous tissue of rabbit models, and at 4, 26, and 52 weeks after implantation, biopsy samples were collected for hematoxylin and eosin and Masson's trichrome staining to evaluate differences in the tissue response between different implants.
The results of in vitro research demonstrated that while the addition of short-chain hydrophilic PEG afforded a smoother surface for the microspheres, it had no significant effect on the molecular weight and degradation rate of PLLA. The histological examination revealed that the PLLA-b-PEG microspheres exhibited enhanced biocompatibility compared with the pure PLLA microspheres, while the irregular PLLA particles showed the highest inflammatory response among the 3 materials.
In this study, we found that the properties of PLLA were improved upon modification by short-chain PEG without reducing the collagen regeneration ability, thereby affording a better histocompatibility.
可生物降解聚合物常用于整形外科的真皮填充剂。其中，聚-L-乳酸（PLLA）因其良好的生物相容性、可降解性以及作为胶原蛋白刺激剂的能力而脱颖而出。
在本研究中，对具有不同微观形态和表面亲水性的PLLA微球在体外和体内的差异行为进行了研究。
将短链亲水性聚乙二醇（PEG）引入PLLA分子中，以改变微球的形态并增强其表面亲水性。对PLLA和PLLA-b-PEG微球的形态和理化性质进行了表征。将不规则PLLA颗粒、PLLA和PLLA-b-PEG微球植入兔模型的皮下组织，在植入后4周、26周和52周采集活检样本，进行苏木精-伊红染色和Masson三色染色，以评估不同植入物之间组织反应的差异。
体外研究结果表明，添加短链亲水性PEG使微球表面更光滑，但对PLLA的分子量和降解速率没有显著影响。组织学检查显示，与纯PLLA微球相比，PLLA-b-PEG微球表现出更强的生物相容性，而不规则PLLA颗粒在这三种材料中表现出最高的炎症反应。
在本研究中，我们发现通过短链PEG改性后，PLLA的性能得到改善，同时不降低其胶原蛋白再生能力，从而具有更好的组织相容性。
