Yang Wei, Niu Huanyun, He Tong, Zhang Zilin, Wang Shiwei, Ren Shuang, Wang Lin
Aesthet Surg J Open Forum. 2024 Nov 7;6:ojae100. doi: 10.1093/asjof/ojae100. eCollection 2024.
Wrinkles and sagging, characteristics of aging, are associated with reductions in collagen and fat. Poly-L-lactic acid (PLLA) is widely used clinically as a tissue filler owing to its good biocompatibility and ability to improve wrinkles and signs of aging. Despite extensive studies of the mechanism of action of PLLA when used as a dermal filler, few studies have examined its effects on adipose tissue.
The short-term effects of PLLA-b-polyethylene glycol (PEG) microspheres implanted in subcutaneous back adipose tissue (BAT) and visceral epididymal adipose tissue (EAT) of rats were examined.
The authors divided 15 male Sprague-Dawley rats into 5 groups based on implantation time, and PLLA-b-PEG microspheres were implanted into the BAT (3 groups were sampled at 6, 8, and 12 weeks) and EAT (2 groups were sampled at 6 and 12 weeks) of rats. Tissue samples were collected at different time points postimplantation and subjected to histological analyses using hematoxylin and eosin, Masson's trichrome, and immunofluorescence staining.
Implantation of PLLA-b-PEG microspheres into different adipose tissues resulted in a mild and persistent inflammatory reaction, increased fibrous connective tissue, and noticeable collagen regeneration. Immunofluorescence showed the upregulation of uncoupling protein (UCP) 1 and UCP2 in the visceral adipose tissue surrounding the implant.
PLLA-b-PEG microspheres exhibited good tissue compatibility and induced an increase in fibrous connective tissue postimplantation, potentially mitigating oxidative damage and improving adipose tissue quality.
皱纹和皮肤松弛是衰老的特征，与胶原蛋白和脂肪减少有关。聚-L-乳酸（PLLA）因其良好的生物相容性以及改善皱纹和衰老迹象的能力，在临床上被广泛用作组织填充剂。尽管对PLLA用作真皮填充剂时的作用机制进行了广泛研究，但很少有研究考察其对脂肪组织的影响。
研究聚-L-乳酸-b-聚乙二醇（PLLA-b-PEG）微球植入大鼠背部皮下脂肪组织（BAT）和附睾内脏脂肪组织（EAT）后的短期效果。
作者根据植入时间将15只雄性Sprague-Dawley大鼠分为5组，将PLLA-b-PEG微球植入大鼠的BAT（3组分别在6周、8周和12周取样）和EAT（2组分别在6周和12周取样）。在植入后的不同时间点收集组织样本，并用苏木精-伊红染色、Masson三色染色和免疫荧光染色进行组织学分析。
将PLLA-b-PEG微球植入不同脂肪组织会导致轻度且持续的炎症反应、纤维结缔组织增加以及明显的胶原再生。免疫荧光显示植入物周围内脏脂肪组织中解偶联蛋白（UCP）1和UCP2上调。
PLLA-b-PEG微球表现出良好的组织相容性，植入后可诱导纤维结缔组织增加，可能减轻氧化损伤并改善脂肪组织质量。
