College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.
Gansu Bailu Grass Industry Development Co., Ltd., Lanzhou, Gansu, China.
PeerJ. 2024 Nov 25;12:e18630. doi: 10.7717/peerj.18630. eCollection 2024.
Whole-plant corn silage (WPCS) is an important roughage source for livestock, and have critical influences on rumen or intestinal microbiota, thus affecting the growth performance and feed efficiency. Our previous studies showed that adding WPCS to the diet of Hezuo pigs could promote the growth and fiber digestibility. While the aim of this study is to understand the effect of dietary WPCS on the colonic microflora in Hezuo pigs, which is essential for improving the roughage exploitation of pigs.
Thirty-two Hezuo pigs with similar body weight (7.88 ± 0.81) kg were selected and randomly divided into four groups with eight pigs in each group. Pigs in the control group were fed a basal diet, pigs in the experimental groups (Groups I, II, and III) were fed basal diet supplemented with 5%, 10%, and 15% WPCS, respectively, under 120 d experimental period. Six pigs from each group were picked for collecting colonic contents samples. 16S rRNA sequencing was performed to analyze the colonic microbiota of experimental pigs.
The results showed that community richness indexes Chao1 and Observed_species in group III of Hezuo pig were significantly lower than that of the other three groups, community diversity indexes Shannon and Simpson were significantly higher in group I and II in comparison to the control group, and significantly lower in group III in comparison to the control group, group I and II. Adding WPCS to the diet of Hezuo pigs has no influence on the colonic dominant phylum, and were most prevalent in the colon of Hezuo pig. When compared with the control group, the relative abundance of was significantly decreased in three experimental groups, while , , , , and exhibited significantly higher relative abundances in at least two experimental groups. , in group I, in group II, in group III exhibited increased relative abundance as compared with the control group. PICRUSt functional annotation indicated that the functions of cellular process and signaling were significantly increased in all WPCS-rationed groups, cancers, nervous system, immune system and environmental adaptation were all differed from groups I and II; three predominant pathways of translation, nucleotide metabolism and signal were only differed from the group II.
Feeding with 5% and 10% WPCS for Hezuo pigs could improve their colonic microflora diversity, and increase the relative abundance of fiber-digesting bacteria, which may potentially help to improve the fibre digestibility of Hezuo pigs by regulating the microbial function of cellular process and signaling, nucleotide metabolism, translation.
全株玉米青贮（WPCS）是家畜重要的粗饲料来源，对瘤胃或肠道微生物群有重要影响，从而影响生长性能和饲料效率。我们之前的研究表明，在合作猪的饮食中添加 WPCS 可以促进生长和纤维消化。本研究旨在了解日粮 WPCS 对合作猪结肠微生物群的影响，这对于提高猪对粗饲料的利用至关重要。
选择 32 头体重（7.88±0.81）kg 相近的合作猪，随机分为 4 组，每组 8 头。对照组猪饲喂基础日粮，试验组（I、II 和 III 组）分别在基础日粮中添加 5%、10%和 15%的 WPCS，试验期为 120d。每组随机抽取 6 头采集结肠内容物样品。采用 16S rRNA 测序技术分析试验猪的结肠微生物群。
结果表明，合作猪 III 组的群落丰富度指数 Chao1 和 Observed_species 显著低于其他 3 组，I 组和 II 组的群落多样性指数 Shannon 和 Simpson 显著高于对照组，III 组显著低于对照组和 I 组和 II 组。在合作猪的日粮中添加 WPCS 对结肠优势门无影响，最常见的是合作猪的结肠。与对照组相比，三个试验组中相对丰度显著降低，而、、、、和在至少两个试验组中表现出相对丰度显著升高。I 组中的、II 组中的、III 组中的相对丰度与对照组相比均增加。PICRUSt 功能注释表明，所有 WPCS 喂养组的细胞过程和信号功能显著增加，癌症、神经系统、免疫系统和环境适应均与 I 组和 II 组不同；三个主要途径翻译、核苷酸代谢和信号仅与 II 组不同。
合作猪日粮中添加 5%和 10%的 WPCS 可改善其结肠微生物群多样性，并增加纤维分解菌的相对丰度，这可能通过调节细胞过程和信号、核苷酸代谢、翻译的微生物功能，有助于提高合作猪的纤维消化率。
