Holahan P K, Dewey W C
Radiat Res. 1986 Apr;106(1):111-21.
Synchronous Chinese hamster ovary cells were heated in G1 and incubated at 37 degrees C at pH 6.75 or pH 7.4 before they were heated a second time. The magnitude and rate of development and decay of thermotolerance were greatly reduced at pH 6.75. This was also observed for asynchronous cells. Furthermore, the heat-induced delay in cell cycle progression was greatly enhanced at low pH and correlated with the reduced rate for development and decay of thermotolerance. However, studies with [3H]TdR to kill cells entering S phase showed that the decay of thermotolerance is relatively independent of the cell cycle. Therefore, low pH apparently slows many cell processes, including those associated with heat-induced cell cycle delay and the rate of development and decay of thermotolerance.
同步化的中国仓鼠卵巢细胞在G1期受热，然后在37℃、pH 6.75或pH 7.4条件下孵育，之后再次受热。在pH 6.75时，热耐受性的发展程度和速率以及衰退程度都大大降低。异步细胞也观察到了这种情况。此外，在低pH条件下，热诱导的细胞周期进程延迟大大增强，并且与热耐受性发展和衰退速率的降低相关。然而，用[3H]TdR杀死进入S期的细胞的研究表明，热耐受性的衰退相对独立于细胞周期。因此，低pH显然会减缓许多细胞过程，包括与热诱导的细胞周期延迟以及热耐受性发展和衰退速率相关的过程。
