Chen Yundong, Luo Wencong, Hu Mingyue, Yao Xiaoyu, Wang Jishi, Huang Yi
Department of Hematopathology, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China.
Department of Clinical Medicine, Guizhou Medical University, Guiyang, Guizhou 550025, P.R. China.
Oncol Lett. 2024 Nov 19;29(1):62. doi: 10.3892/ol.2024.14808. eCollection 2025 Jan.
Acute myeloid leukemia (AML) is a hematological cancer prevalent worldwide. Anoikis-related genes (ARGs) are crucial in the progression of cancer and metastasis of tumors. However, their role in AML needs to be clarified. In the present study, differential analysis was performed on data from The Cancer Genome Atlas database to identify differentially expressed ARGs (DE-ARGs). Subsequently, a prognostic model for patients with AML was constructed using univariate Cox, Least Absolute Shrinkage and Selection Operator and multivariate Cox regression analyses. This model was based on four key DE-ARGs [lectin galactoside-binding soluble 1 (LGALS1), integrin subunit α 4 (ITGA4), hepatocyte growth factor (HGF) and Ras homolog gene family member C (RHOC)]. Independent prognostic factors for AML included prior treatment, age, risk scores and diagnosis. A nomogram was constructed based on these factors to aid clinical decision-making. Furthermore, bone marrow samples were collected from individuals diagnosed with AML and healthy donors to validate the expression of the identified ARGs using reverse transcription-quantitative PCR. The mRNA levels of LGALS1 and RHOC were significantly higher, while those of ITGA4 and HGF were significantly lower in patients with AML than in healthy donors (all P<0.05). The results of the present study expands the understanding of the function of ARGs in AML, providing a new theoretical basis for the treatment of AML.
急性髓系白血病（AML）是一种在全球范围内普遍存在的血液系统癌症。失巢凋亡相关基因（ARGs）在癌症进展和肿瘤转移中起着关键作用。然而，它们在AML中的作用尚需阐明。在本研究中，对来自癌症基因组图谱数据库的数据进行差异分析，以鉴定差异表达的ARGs（DE-ARGs）。随后，使用单变量Cox回归、最小绝对收缩和选择算子以及多变量Cox回归分析，为AML患者构建了一个预后模型。该模型基于四个关键的DE-ARGs[半乳糖凝集素1（LGALS1）、整合素亚基α4（ITGA4）、肝细胞生长因子（HGF）和Ras同源基因家族成员C（RHOC）]。AML的独立预后因素包括既往治疗、年龄、风险评分和诊断。基于这些因素构建了列线图，以辅助临床决策。此外，从诊断为AML的个体和健康供体中采集骨髓样本，使用逆转录定量PCR验证所鉴定ARGs的表达。与健康供体相比，AML患者中LGALS1和RHOC的mRNA水平显著升高，而ITGA4和HGF的mRNA水平显著降低（均P<0.05）。本研究结果扩展了对ARGs在AML中功能的认识，为AML的治疗提供了新的理论依据。
