Post-Doctoral Fellow at College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA.
Associate Professor of Large Animal Internal Medicine, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Vet Med Sci. 2025 Jan;11(1):e70142. doi: 10.1002/vms3.70142.
During the transitional period, dairy cows experience oxidative stress and are more susceptible to diseases, including left displacement of the abomasum (LDA).
This study aimed to compare oxidative stress biomarker levels in cows with LDA to those in healthy conditions and investigate the associations between predictive metabolites linked to LDA and oxidative stress biomarkers.
In this case-control study, 400 healthy multiparous Holstein cows were matched for lactation number, milk production and calving date. Blood samples were collected at four time points: 21 and 7 days before, as well as 7 and 21 days after parturition from all animals. During the observation period, seven cows diagnosed with LDA in the main population, and seven healthy cows were randomly selected as controls for the comparison of oxidative stress, liver enzymes and metabolic parameters. Analysis of blood parameters utilized repeated measures ANOVA, and the degree of relationship between oxidative stress biomarkers and other measured parameters was assessed using Pearson correlations.
The LDA group exhibited significantly higher levels of urea, blood urea nitrogen (BUN), gamma-glutamyl transferase, glucose, cholesterol, triglyceride (TAG), β-hydroxybutyric acid (BHBA), aspartate aminotransferase (AST), sorbitol dehydrogenase, serum amyloid A (SAA), chloride, sodium, potassium, total antioxidant capacity (TAC) and malondialdehyde (MDA) compared to the control cows (p < 0.05). Positive correlations were observed among BUN, glucose, TB, AST, SAA, BHBA, TAG and MDA. Conversely, these parameters displayed negative correlations with TAC. Negative correlations were found among chloride, sodium, potassium, calcium, phosphorus and MDA, whereas positive correlations were observed with TAC.
These findings highlight the elevated level of oxidative stress and compromised antioxidant defence in cows with LDA and the intricate interplay among oxidative stress, metabolic parameters and liver enzymes.
在过渡期，奶牛会经历氧化应激，更容易患病，包括真胃左方变位（LDA）。
本研究旨在比较 LDA 奶牛与健康奶牛的氧化应激生物标志物水平，并探讨与 LDA 相关的预测代谢物与氧化应激生物标志物之间的关系。
在这项病例对照研究中，我们匹配了 400 头健康的经产荷斯坦奶牛的泌乳次数、产奶量和产犊日期。所有动物在四个时间点采集血液样本：分娩前 21 天和 7 天，以及分娩后 7 天和 21 天。在观察期间，从主要人群中诊断出 7 头患有 LDA 的奶牛，并随机选择 7 头健康奶牛作为对照组，比较氧化应激、肝酶和代谢参数。采用重复测量方差分析分析血液参数，采用 Pearson 相关评估氧化应激生物标志物与其他测量参数之间的关系程度。
LDA 组的尿素、血尿素氮（BUN）、γ-谷氨酰转移酶、葡萄糖、胆固醇、甘油三酯（TAG）、β-羟丁酸（BHBA）、天门冬氨酸氨基转移酶（AST）、山梨醇脱氢酶、血清淀粉样蛋白 A（SAA）、氯、钠、钾、总抗氧化能力（TAC）和丙二醛（MDA）水平显著高于对照组（p<0.05）。BUN、葡萄糖、总胆红素、AST、SAA、BHBA、TAG 和 MDA 之间存在正相关。相反，这些参数与 TAC 呈负相关。氯、钠、钾、钙、磷和 MDA 之间存在负相关，而与 TAC 呈正相关。
这些发现强调了 LDA 奶牛氧化应激水平升高和抗氧化防御受损，以及氧化应激、代谢参数和肝酶之间的复杂相互作用。
