使用高压液相色谱法和放射免疫分析法测量生物样品中的前列腺素E3和其他类二十烷酸。
Measurement of prostaglandin E3 and other eicosanoids in biologic samples using high pressure liquid chromatography and radioimmunoassay.
作者信息
Gallon L S, Barcelli U O
出版信息
Prostaglandins. 1986 Feb;31(2):217-25. doi: 10.1016/0090-6980(86)90048-1.
A method to measure PGE3 in biologic samples is described. Complete resolution of PGE3 from PGE1 and PGE2 is achieved by reversephase high pressure liquid chromatography. Quantification is carried out by radioimmunoassay using an antibody directed against PGE2 that has high cross-reactivity with PGE3. Using this method, a marked increase in PGE3 production by mouse kidney tissue and in rat urine was demonstrated after supplemental feeding of omega-3 fatty acids. This method can also be applied to measurement of 6-keto-PGF1 alpha and TXB2 in the same samples.
本文描述了一种测量生物样品中PGE3的方法。通过反相高压液相色谱法可实现PGE3与PGE1和PGE2的完全分离。使用针对PGE2且与PGE3具有高交叉反应性的抗体，通过放射免疫测定法进行定量。使用该方法，在补充ω-3脂肪酸后，小鼠肾组织和大鼠尿液中PGE3的产生显著增加。该方法也可应用于同一样品中6-酮-PGF1α和TXB2的测量。
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