Institute of Chemical Technology and Engineering, Poznan University of Technology, Poznań, Poland.
Institute of Building Engineering, Poznan University of Technology, Poznań, Poland.
J Biomed Mater Res B Appl Biomater. 2024 Dec;112(12):e35517. doi: 10.1002/jbm.b.35517.
This study presents a new innovative drug delivery system for ciprofloxacin, which is based on the formation of a zinc-doped carbon dots layer on the surface of a titanium alloy (TiAl4V6). In the study, the effectiveness of the synthesis method of a zinc-doped carbon dots layer was determined. The distribution of the layer of carbon dots on the surface of the titanium alloy was investigated using the FT-IR mapping technique, which confirmed the efficiency of the synthesis. The effective synthesis of carbon dots and the coordination of zinc ions on their surface opens the possibility of sorption of ciprofloxacin, which results in a high application potential of the obtained biomaterial. The introduction of zinc cations on the surface of the carbon dots layer resulted in high sorption results of the active substance (40 μg of drug per 1 cm of implant). The release profile of ciprofloxacin from the modified surface of the titanium alloy indicates that this active substance can be released for up to 4 h. The biomaterial obtained in this work is also hydrophilic (about 40°), which was shown by the contact angle tests. This is an important feature and indicates a high application potential of the performed modification. The resulting layer has antibacterial properties. Growth inhibition for microorganisms such as Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Candida albicans ranged from 74% to 96%. The creation of such a layer on the titanium alloy may reduce the risk of infection during the implantation procedure.
本研究提出了一种新的环丙沙星递药系统，该系统基于在钛合金（TiAl4V6）表面形成锌掺杂碳点层。在研究中，确定了锌掺杂碳点层的合成方法的有效性。使用 FT-IR 映射技术研究了碳点层在钛合金表面的分布，证实了合成的效率。碳点的有效合成以及锌离子在其表面的配位为环丙沙星的吸附开辟了可能性，这使得所获得的生物材料具有很高的应用潜力。锌阳离子在碳点层表面的引入导致活性物质（每 1cm 植入物 40μg 药物）的高吸附效果。从钛合金改性表面释放环丙沙星的曲线表明，这种活性物质可以释放长达 4 小时。本工作中获得的生物材料也具有亲水性（约 40°），这通过接触角测试得到了证实。这是一个重要的特征，表明所进行的修饰具有很高的应用潜力。所得的层具有抗菌性能。对铜绿假单胞菌、大肠杆菌、金黄色葡萄球菌、蜡状芽孢杆菌和白色念珠菌等微生物的生长抑制率在 74%至 96%之间。在钛合金上形成这样的层可能会降低植入过程中感染的风险。
