Xu Junpeng, Jia Qingge, Du Na, Liang Jiayi, Wang Huanhuan, Chai Jia, Yang Yanru, Xu Tianqi, Ma Jianwu, Ji Puzhong, Ma Yingchun, Liu Bin, Yang Weiyi, Ma Jing, Yang Yanli, Li Mingyang
Department of Pathology, The 940th Hospital of the Joint Logistics Support Force, Lanzhou, China.
Department of Reproductive Medicine, Xi'an International Medical Center Hospital, Northwest University, Xi'an, China.
Pathol Res Pract. 2025 Jan;265:155744. doi: 10.1016/j.prp.2024.155744. Epub 2024 Nov 28.
Esophageal squamous cell carcinoma (ESCC) is a malignant tumor that poses a significant threat to human health. Patients are often diagnosed at advanced stages of the disease, resulting in poor clinical outcomes and a short survival period. Recent advances have revealed that ESCC tumors exhibit distinct molecular biological characteristics. Our study investigated the expression and biological function of Stanniocalcin-1 (STC1) in ESCC.
We collected paraffin-embedded tumor tissues from 127 patients with ESCC at Xijing Hospital, as well as fresh tissue specimens from 21 patients who underwent radical resection of ESCC, including both tumor and adjacent normal tissues. The expression levels of STC1 in ESCC tissues and cells were assessed using immunohistochemistry (IHC) and Western blot analysis. We employed Kaplan-Meier survival analysis to explore the impact of STC1 expression on the prognosis of ESCC patients. Additionally, we evaluated the effect of STC1 expression on the malignant behavior of ESCC cells through both in vivo and in vitro experiments.
Compared to normal esophageal tissue, STC1 is overexpressed in ESCC tissue. Univariate and multivariate analyses of clinical data indicated that patients with STC1 overexpression had a poor prognosis (P = 0.009 and P = 0.015). Both cell experiments and xenograft models demonstrated that the upregulation of STC1 may promote the malignant behavior of ESCC, and conversely, its downregulation may inhibit such behavior.
The overexpression of STC1 enhances the migration, invasion and proliferation of ESCC cells, and is significantly associated with poor prognosis in ESCC patients. Therefore, STC1 may serve as a promising prognostic factor and could also be a potential target for ESCC-specific therapy.
食管鳞状细胞癌（ESCC）是一种对人类健康构成重大威胁的恶性肿瘤。患者常被诊断为疾病晚期，导致临床预后不良和生存期短。最近的进展表明，ESCC肿瘤具有独特的分子生物学特征。我们的研究调查了Stanniocalcin-1（STC1）在ESCC中的表达及生物学功能。
我们收集了西京医院127例ESCC患者的石蜡包埋肿瘤组织，以及21例行ESCC根治性切除术患者的新鲜组织标本，包括肿瘤组织和相邻正常组织。采用免疫组织化学（IHC）和蛋白质印迹分析评估ESCC组织和细胞中STC1的表达水平。我们采用Kaplan-Meier生存分析来探讨STC1表达对ESCC患者预后的影响。此外，我们通过体内和体外实验评估STC1表达对ESCC细胞恶性行为的影响。
与正常食管组织相比，STC1在ESCC组织中过表达。临床数据的单因素和多因素分析表明，STC1过表达的患者预后较差（P = 0.009和P = 0.015）。细胞实验和异种移植模型均表明，STC1的上调可能促进ESCC的恶性行为，反之，其下调可能抑制这种行为。
STC1的过表达增强了ESCC细胞的迁移、侵袭和增殖能力，并且与ESCC患者的不良预后显著相关。因此，STC1可能是一个有前景的预后因素，也可能是ESCC特异性治疗的潜在靶点。
